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Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Electrophoresis: Overview01:20

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

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Updated: Jun 3, 2026

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
08:31

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

Published on: October 15, 2013

Field-inversion gel electrophoresis.

G F Carle, G F Carle

    Methods in Molecular Biology (Clifton, N.J.)
    |March 17, 2011
    PubMed
    Summary
    This summary is machine-generated.

    Field-inversion gel electrophoresis (FIGE) offers an accessible method for separating large DNA fragments using standard equipment. This technique simplifies DNA fragment analysis by employing a two-electrode system for uniform electric fields.

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    Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
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    Published on: October 15, 2013

    Agarose Gel Electrophoresis for the Separation of DNA Fragments
    07:10

    Agarose Gel Electrophoresis for the Separation of DNA Fragments

    Published on: April 20, 2012

    CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis
    11:38

    CN-GELFrEE - Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis

    Published on: February 29, 2016

    Area of Science:

    • Molecular Biology
    • Biotechnology
    • Genetics

    Background:

    • Separating large DNA fragments is crucial for various molecular biology applications.
    • Existing methods like pulsed-field gel electrophoresis (PFGE) can require specialized equipment.
    • Field-inversion gel electrophoresis (FIGE) emerged as a simpler alternative.

    Purpose of the Study:

    • To introduce and describe the Field-Inversion Gel Electrophoresis (FIGE) technique.
    • To highlight the simplicity and minimal equipment requirements of FIGE.
    • To explain the underlying principles of FIGE derived from orthogonal-field-alternation gel electrophoresis (OFAGE).

    Main Methods:

    • Utilizes a standard gel electrophoresis box and power supply.
    • Incorporates a device for periodic inversion of the electric field direction.
    • Adapts principles from OFAGE, reducing a four-electrode system to two electrodes for a uniform electric field.

    Main Results:

    • FIGE is demonstrated as an easy-to-perform technique for large DNA fragment separation.
    • The method requires only basic gel electrophoresis apparatus.
    • A highly uniform electric field is generated, facilitating easy lane-to-lane comparisons.

    Conclusions:

    • Field-inversion gel electrophoresis (FIGE) provides a straightforward and accessible approach for separating large DNA fragments.
    • The technique's simplicity and reliance on standard equipment make it a practical option for researchers.
    • FIGE's uniform electric field enhances the reliability of DNA fragment analysis.