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KIAA1429 promotes gastric cancer progression by destabilizing RASD1 mRNA in an m6A-YTHDF2-dependent manner
Mengting Ren1, Hanghai Pan1, Xinxin Zhou2
1Cancer Center, Department of Gastroenterology, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou, Zhejiang, China.
Journal of Translational Medicine
|June 20, 2024
Summary
KIAA1429 promotes gastric cancer (GC) by destabilizing RASD1 mRNA, leading to increased tumor growth and metastasis. This finding establishes KIAA1429 as a potential prognostic biomarker and therapeutic target for GC patients.
Area of Science:
- Oncology
- Molecular Biology
- Epigenetics
Background:
- KIAA1429 is a key component of the m6A methyltransferase complex, linked to various cancer progressions.
- Its specific role and mechanisms in gastric cancer (GC) remain largely uncharacterized.
Purpose of the Study:
- To investigate the function of KIAA1429 in gastric cancer.
- To elucidate the molecular mechanisms underlying KIAA1429's role in GC progression.
Main Methods:
- Quantitative real-time PCR, Western blotting, and immunohistochemistry were used to assess KIAA1429 expression.
- In vitro and in vivo functional assays, alongside m6A-related techniques (MeRIP-seq, RIP, RNA pull-down), were employed to determine mechanisms.
Main Results:
- KIAA1429 expression is elevated in GC tissues and correlates with poor prognosis.
- KIAA1429 promotes GC cell proliferation, migration, invasion, and tumor growth/metastasis.
- KIAA1429 upregulates RASD1 by increasing m6A modification and enhancing mRNA stability in an m6A-YTHDF2-dependent manner.
Conclusions:
- KIAA1429 acts as an oncogene in GC by downregulating RASD1 via m6A-YTHDF2-mediated mRNA destabilization.
- KIAA1429 presents potential as a prognostic biomarker and therapeutic target for gastric cancer.
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