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Updated: Jul 10, 2026

Seawater Sampling and Collection

Seawater Sampling and Collection

Published on: June 17, 2009

Marine eDNA sampling from submerged surfaces with paint rollers.

Simon Jarman¹, Jason B Alexander², Kathryn L Dawkins³

¹School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia; eDNA Frontiers, Curtin University, Bentley, WA, Australia.

Marine Genomics

|June 21, 2024

Summary

Environmental DNA (eDNA) sampling is key for marine biodiversity. Microfibre paint rollers offer a rapid, affordable, and effective method for collecting underwater eDNA, improving species detection rates.

Keywords:

Biodiversity Detection Environmental DNA Invasive marine species Metabarcoding

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Area of Science:

Marine biology
Environmental science
Molecular ecology

Background:

Environmental DNA (eDNA) analysis is a powerful tool for measuring marine biodiversity.
Effective eDNA sampling methods are crucial for improving species detection rates and survey outcomes.
Current methods require optimization for speed, cost-effectiveness, versatility, and practicality.

Purpose of the Study:

To compare the effectiveness of different methods for sampling eDNA from submerged marine surfaces.
To identify a rapid, affordable, versatile, and practical eDNA sampling method for marine environments.

Main Methods:

Comparison of eDNA detection using polyurethane foam, nylon swabs, microfibre paint rollers, and sediment scoops.
Analysis of species diversity from samples collected from submerged marine surfaces.
Assessment of method practicality, affordability, and versatility.

Main Results:

All tested methods successfully detected a diverse range of species (>250 multicellular species).
Microfibre paint rollers proved to be an effective, readily available, and affordable sampling method.
This method facilitates eDNA sampling from submerged marine surfaces, even in difficult-to-reach areas.

Conclusions:

Microfibre paint rollers represent a practical and cost-effective advancement in marine eDNA sampling techniques.
Wider application of this method can enhance the scope and efficiency of marine biodiversity surveys.
Improved sampling methods contribute to more comprehensive detection of marine organisms.