Development of bio sheet using nanoparticles and the confederation of protein for casting wound

This research pioneers a sustainable approach to wound healing by developing a bio sheet for dressings. It ingeniously incorporates healing properties of reduced graphene oxide synthesized via an eco-friendly method, using phytoextracts to eliminate toxic chemicals. Collagen extracted from fish waste and fibrinogen from post-animal waste contribute to the bio sheet's green profile. The study emphasizes the global shift towards utilizing natural materials in therapeutic applications for efficient wound healing while minimizing toxic side effects. The formulation involves a simple muffle treatment for the conversion of carbon to graphene oxide, followed by a greener reduction technique to synthesize rGO. Characterization- by peaks that obtained at 255 nm, 240 nm, 280 nm, 360 nm using UV-Vis analysis were confirmed the presents of desired carbon and glycoproteins, In X-ray diffraction (2θ value) we found peaks at 24.37°, 27.84°, 17.43° which indicated the presents of rGO, Fibrinogen, and Collagen, and FT-IR methods confirms the functional groups like OH, CH², CC, CHO and NC in the derived materials and Zeta potential distribution were done for Compounds. To add up purity and efficiency analysis via GCMS were done for proteins. The resulting bio sheet, created in a controlled environment with a gelling agent, Undergoes morphological analysis through FESEM-EDX. Additional assessments, including antioxidant, anti-inflammatory, and hemocompatibility assays, illuminate the nature of the formulated bio sheet. To addition, biosheet is analyzed for physical and mechanical properties. Further insight is gained through the analysis of surface plots of the EDX images of both the composite and bio sheet using ImageJ software. This comprehensive approach underscores the potential of sustainable and naturally derived materials in advancing wound care technologies.