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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

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Related Experiment Video

Updated: Jun 23, 2025

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

Published on: October 27, 2011

Short-Read RNA-Seq.

Rong Hu¹, Md N Islam¹, Rency S Varghese¹

¹Genomics & Epigenomics Shared Resource, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, USA.

Methods in Molecular Biology (Clifton, N.J.)

|June 22, 2024

Summary

This summary is machine-generated.

This guide details RNA sequencing (RNA-Seq) methods for analyzing transcriptomes. It covers RNA enrichment, library preparation, and sequencing, emphasizing quality control for accurate gene expression studies.

Keywords:

Library preparation RNA-Seq Short-read sequencing

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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Area of Science:

Molecular Biology
Genomics
Bioinformatics

Background:

RNA sequencing (RNA-Seq) is a key technology for transcriptome analysis.
It enables the study of gene expression, copy number variation, alternative splicing, and novel transcripts.

Purpose of the Study:

To provide a comprehensive methodology for short-read RNA-Seq.
To offer practical guidance and best practices for optimizing the RNA-Seq workflow.
To highlight critical quality control steps for high-quality data.

Main Methods:

RNA enrichment and quality assessment.
Library preparation protocols for short-read sequencing.
Sequencing techniques and data generation.

Main Results:

Detailed step-by-step protocols for RNA-Seq.
Practical tips for workflow optimization.
Identification of crucial quality control checkpoints.

Conclusions:

Successful RNA-Seq requires meticulous attention to each workflow step.
Implementing quality control measures is essential for reliable transcriptome analysis.
This chapter serves as a practical resource for researchers conducting RNA-Seq.