High methane potential of oxygenic photogranules decreases after starvation

Oxygenic photogranules (OPG) are granular biofilms that can treat wastewater without external aeration, making it an advantage over activated sludge. Excess of OPG biomass can serve as energy source through anaerobic digestion. Two sequencing batch photoreactors were operated over 400 days to grow OPG. Biochemical methane potentials (BMP) were obtained from near-infrared spectroscopy. OPGs had an average BMP of 356 mL CH₄·gVS^-1, much higher than typical BMP from activated sludge. A partial least squares analysis could relate BMP with reactor operating conditions, like light regime, load or biomass concentration. Since organic load was the most influential parameter on BMP, three starvation experiments were set up. An average decrease of BMP by 18.4 % was observed. However, the unexpected growth of biomass during starvation resulted in a higher total methane volume. In conclusion, starvation reduces the BMP of OPGs but anaerobic digestion of OPG biomass remains a promising route for biomass valorization.