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Updated: Jun 23, 2025
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Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy
Published on: March 24, 2014
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CD147 regulates the formation and function of immune synapses.
Yingming Xu1, Kui Zhang1, Jinlin Miao1
1Department of Clinical Immunology, Xijing Hospital, and Department of Cell Biology of National Translational Science Center for Molecular Medicine, Fourth Military Medical University, Xi'an, China.
Cellular Immunology
|June 23, 2024
Summary
CD147 is crucial for immune synapse formation and T cell activation. Its presence in the peripheral super molecular cluster (p-SMAC) regulates T cell receptor signaling and IL-2 secretion.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- CD147 is a molecule associated with T cell activation.
- Its exact role in T cell activation and immune synapse (IS) formation is not fully understood.
Purpose of the Study:
- To elucidate the precise role of CD147 in T cell activation and IS formation.
- To investigate the molecular mechanisms underlying CD147's function in T cell signaling.
Main Methods:
- Studied CD147 translocation to the IS upon T cell activation.
- Utilized CD147 knockdown in T cells to assess its impact on IS formation.
- Investigated the effect of CD147 ligation with mAb HAb18 on T cell activation and IL-2 secretion.
- Analyzed the distribution of CD98 and phosphorylation of TCR-related molecules (ZAP-70, ERK, cJun).
Main Results:
- CD147 translocated to the IS, primarily in the p-SMAC, during T cell activation.
- CD147 knockdown in T cells impaired IS formation with antigen-presenting cells (APCs).
- CD147 ligation inhibited T cell activation and IL-2 secretion.
- CD98 distribution in the IS was CD147-dependent, and CD147 ligation reduced phosphorylation of TCR signaling molecules.
Conclusions:
- CD147 is indispensable for immune synapse formation.
- CD147 plays a significant role in regulating T cell activation and function through TCR signaling pathways.
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