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Updated: Jul 23, 2026

Protocol to Create Chronic Wounds in Diabetic Mice
Published on: September 25, 2019
Modulation of diabetic wound healing using carbon monoxide gas-entrapping materials
Emily Witt1,2,3, Alexander J Leach3,4, Jianling Bi1,2,3
1Department of Biomedical Engineering, University of Iowa, Iowa City, IA, 52242, USA.
Abstract:
Diabetic wound healing is uniquely challenging to manage due to chronic inflammation and heightened microbial growth from elevated interstitial glucose. Carbon monoxide (CO), widely acknowledged as a toxic gas, is also known to provide unique therapeutic immune modulating effects. To facilitate delivery of CO, we have designed hyaluronic acid-based CO-gas-entrapping materials (CO-GEMs) for topical and prolonged gas delivery to the wound bed. We demonstrate that CO-GEMs promote the healing response in murine diabetic wound models (full-thickness wounds and pressure ulcers) compared to N2-GEMs and untreated controls.
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