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Related Concept Videos

Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
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Related Experiment Video

Updated: Jun 23, 2025

Author Spotlight: Analyzing the Synaptic Ultrastructure in Mature Retinal Organoids Using TEM
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Author Spotlight: Analyzing the Synaptic Ultrastructure in Mature Retinal Organoids Using TEM

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Preparing Retinal Organoid Samples for Transmission Electron Microscopy.

Xiaoqing Liu1, Bilin Rao2, Qingyang Lin2

  • 1State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University; Laboratory of Retinal Physiology and Disease, School of Ophthalmology and Optometry, Wenzhou Medical University; q15771553706@163.com.

Journal of Visualized Experiments : Jove
|June 24, 2024
PubMed
Summary

Researchers developed a simple protocol for preparing retinal organoid (RO) samples for transmission electron microscopy (TEM). This method allows for high-resolution imaging of synaptic structures in ROs, advancing retinal research.

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Area of Science:

  • * Stem Cell Biology
  • * Neuroscience
  • * Ophthalmology

Background:

  • * Retinal organoids (ROs) derived from induced pluripotent stem cells (iPSCs) model human retinal development.
  • * Synapse development in ROs is known, but ultrastructural details remain limited.
  • * Transmission electron microscopy (TEM) is crucial for high-resolution ultrastructural analysis.

Purpose of the Study:

  • * To develop a simple, repeatable protocol for preparing RO samples for TEM.
  • * To enable nanoscale visualization of synaptic contacts in ROs.
  • * To provide high-quality microscopic evidence of RO synaptic ultrastructure.

Main Methods:

  • * Detailed protocol for RO TEM sample preparation.
  • * Steps include fixation, post-fixation, embedding, and visualization.
  • * Focus on achieving high-resolution imaging of synaptic structures.

Main Results:

  • * A simple and repeatable RO TEM sample preparation protocol was established.
  • * The method facilitates high-quality microscopic evidence of RO synaptic contacts.
  • * Enables nanoscale exploration of synaptic structures in ROs.

Conclusions:

  • * The developed protocol is effective for RO TEM sample preparation.
  • * This method enhances the study of synaptic ultrastructure in retinal organoids.
  • * Facilitates deeper understanding of retinal development and synapse maturation in vitro.