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FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Updated: Jun 23, 2025

Robust 3D DNA FISH Using Directly Labeled Probes

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Published on: August 15, 2013

Quantitative FISHing: Implications for Chromosomal Analysis.

Svetlana G Vorsanova^1,2, Yuri B Yurov^1,2, Ivan Y Iourov^1,2

¹Vorsanova's Laboratory of Molecular Cytogenetics of Neuropsychiatric Diseases, Veltischev Research and Clinical Institute for Pediatrics and Pediatric Surgery of the Pirogov Russian National Research Medical University of the Russian Ministry of Health, Moscow, Russia.

Methods in Molecular Biology (Clifton, N.J.)

|June 24, 2024

Summary

This summary is machine-generated.

Quantitative FISHing (QFISHing) enhances efficiency for cancer chromosome research. This method aids in identifying chromosome loss, amplification, and heteromorphisms, crucial for genome studies.

Keywords:

Chromosomal instability Chromosome abnormalities DNA probes Fluorescence in situ hybridization Interphase fluorescence in situ hybridization Quantification Somatic cells

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Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay

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FISH for Pre-implantation Genetic Diagnosis

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Published on: February 23, 2011

Area of Science:

Genetics
Molecular Biology
Cancer Research

Background:

Fluorescence in situ hybridization (FISH) efficiency is improved by signal quantification.
Quantitative FISH analysis (QFISHing) has diverse applications in cytogenetics and molecular diagnostics.

Purpose of the Study:

To provide a detailed, step-by-step protocol for Quantitative FISHing (QFISHing).
To highlight the utility of QFISHing in cancer chromosome research.

Main Methods:

Development and description of a QFISHing protocol.
Application of QFISHing for analyzing chromosomal abnormalities.

Main Results:

QFishing enables differentiation between chromosome loss and associations.
QFishing detects amplification of chromosomal loci.
QFishing quantifies chromosomal heteromorphisms, aiding in parental origin determination.

Conclusions:

QFishing is a valuable technique for cancer chromosome research.
The described protocol facilitates the implementation of QFISHing.
QFishing has significant applications in genome research and diagnostics.