Evaluation of pertinent parameters of a new identification system for non-enteric gram-negative rods

The API 20 NE (Rapid NFT) system for identification of non-enteric aerobic gram-negative rods was evaluated using 431 strains of species covered by the analytical profile index and previously identified by conventional methods. In an analysis of several parameters, 343 (80%) of the strains were identified correctly with good to excellent probabilities of positive reactions (80.0-99.9%), 190 (55%) of these within 24 h; 74 (17%) yielded primary identifications with lower probabilities. Only 3% of the strains could not be correctly identified. The system is very satisfactory for routine use, but could be improved by modified pre-testing of the strains and by including more species in the profile index.