Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Enhancing photodynamic and chemodynamic therapy efficacy through a novel ROS-amplifying therapeutic platform for breast cancer treatment.

Nanoscale horizons·2026

Same author

Intermittent fasting exacerbates microplastic-induced gut inflammation via Rikenella-mediated regulation of purine metabolism and Th17/Treg balance.

NPJ biofilms and microbiomes·2026

Same author

Recognition and Management of Transfusion-Associated Immune Hemolytic Crisis in Pregnant Patients with Thalassemia: Analysis of Three Cases and Construction of a Clinical Pathway.

International journal of women's health·2026

Same author

Transcending Structural Dependencies: A Tunable Mass Spectrometry-Driven Machine Learning Framework for Genotoxicity Prediction.

Environmental science & technology·2026

Same author

GATA6-CRT axis promotes stress-associated autophagy, EMT, and stemness-associated traits in pancreatic cancer.

Cell death & disease·2026

Same author

Network Toxicology and In Vivo Studies Reveal the Toxicity and Mechanisms of Tributyl Citrate Carried by Microplastics in Promoting Colitis-to-Tumorigenesis Transformation.

Environment & health (Washington, D.C.)·2026

Same journal

Structural Hairpin Anchoring-Mediated TtAgo Activity Regulation for Programmable Biosensing.

Analytical chemistry·2026

Same journal

Digital Revitalization of a Legacy Linear Ion Trap System.

Analytical chemistry·2026

Same journal

An Interface-Regulated Electrochemical Biosensing Platform Based on the Cascade Amplification of Primer Exchange Reaction and CRISPR/Cas12a for Noninvasive Bladder Cancer Diagnosis.

Analytical chemistry·2026

Same journal

Spatially Resolved Diffusion NMR for Structurally Heterogeneous Materials.

Analytical chemistry·2026

Same journal

Direct Whole-Blood Multiplexing of Small Molecules via a Micelle-Enhanced Chemiluminescent Paper Sensor with Mesoporous Silica Membrane.

Analytical chemistry·2026

Same journal

Modeling the Effects of Short-Range Randomness in Packed Sphere Beds.

Analytical chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 23, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

Mass-Spectrometry-Based Assay at Single-Base Resolution for Simultaneously Detecting m⁶A and m⁶Am in RNA.

Qiang Sun^1,2, Haijuan Li¹, Ziwei Lin¹

¹College of Pharmacy, Xuzhou Medical University, Xuzhou 221004, Jiangsu, China.

Analytical Chemistry

|June 24, 2024

Summary

This summary is machine-generated.

This study introduces a mass spectrometry (MS) method for precisely detecting N6-methyladenosine (m6A) and N6,2′-O-dimethyladenosine (m6Am) RNA modifications at single-nucleotide resolution.

More Related Videos

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

Published on: September 16, 2019

Related Experiment Videos

Last Updated: Jun 23, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

Published on: September 16, 2019

Area of Science:

Molecular Biology
Biochemistry
Genomics

Background:

Adenosine methylation, including N6-methyladenosine (m6A) and N6,2′-O-dimethyladenosine (m6Am), is crucial for biological processes.
Existing methods for detecting m6A and m6Am at single-base resolution have limitations.

Purpose of the Study:

To develop and validate a mass spectrometry (MS)-based method for simultaneous detection of m6A and m6Am sites.
To achieve single-nucleotide resolution for targeted RNA fragments without PCR amplification.

Main Methods:

Utilized tandem mass spectrometry (MS/MS) combined with targeted RNA enrichment and enzymatic digestion.
Applied the method to cell and tissue samples for m6A and m6Am site detection.
Validated accuracy using CRISPR/Cas9-mediated knockout of methyltransferases.

Main Results:

Successfully achieved simultaneous determination of m6A and m6Am sites in targeted RNA fragments.
Demonstrated high confidence and accuracy in identifying modification sites.
Verified the method's effectiveness in complex biological samples.

Conclusions:

The developed MS-based method provides a reliable and precise approach for simultaneous m6A and m6Am detection.
This technique offers valuable insights into the functional roles of these RNA modifications.
The method eliminates the need for PCR amplification, simplifying RNA modification analysis.