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Portable Aptasensor Based on Parallel Rolling Circle Amplification for Tumor-Derived Exosomes Liquid Biopsy.
Yaqin He1, Xianghu Zeng1, Ying Xiong1
1Department of Laboratory Medicine, Med+X Center for Manufacturing, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|June 26, 2024
Summary
A novel portable aptasensor enables rapid, label-free detection of tumor-derived exosomes (TEXs) using a handheld fluorometer. This technology shows high sensitivity and specificity, correlating well with clinical diagnostics for lung cancer biomarkers.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Molecular Diagnostics
Background:
- Tumor-derived exosomes (TEXs) are crucial biomarkers for early cancer detection.
- Existing methods for exosome detection are often complex, time-consuming, and require specialized equipment.
- Development of rapid, sensitive, and portable diagnostic tools for TEXs is highly needed.
Purpose of the Study:
- To develop a separation-free and label-free portable aptasensor for the rapid and sensitive analysis of tumor-derived exosomes (TEXs).
- To integrate rolling circle amplification (RCA) with a handheld fluorometer for exosome detection.
- To target lung cancer biomarkers, mucin 1 and programmed cell death ligand 1 (PD-L1), on exosomes.
Main Methods:
- A portable aptasensor system was developed, integrating parallel rolling circle amplification (RCA) reactions.
- Selective binding of aptamers to target biomarkers on exosomes modulated RCA product formation.
- A low-cost, highly sensitive handheld fluorometer detected fluorescence changes from quantum dots (QDs) and methylene blue (MB).
Main Results:
- The aptasensor achieved a limit of detection (LOD) as low as 30 particles/mL for cell-derived exosomes.
- High specificity (93%) and sensitivity (92%) were demonstrated in clinical sample analysis.
- The results showed strong concordance with clinical computed tomography (CT) and pathology findings.
Conclusions:
- The developed portable aptasensor offers a rapid, sensitive, and label-free method for TEXs analysis.
- This technology holds promise for point-of-care diagnostics and early cancer detection.
- The system's performance in clinical samples validates its potential for real-world applications.
