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Updated: Jun 23, 2025

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
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Mutational analysis of Mei5, a subunit of Mei5-Sae3 complex, in Dmc1-mediated recombination during yeast meiosis

Stephen Mwaniki1, Priyanka Sawant, Osaretin P Osemwenkhae1

  • 1Division of Integrated Protein Functions, Institute for Protein Research, Osaka University, Suita, Osaka, Japan.

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
|June 26, 2024
PubMed

Insights

The Mei5-Sae3 complex is vital for DNA repair during meiosis. Specific mutations reveal Arg97 is crucial for complex formation and that Mei5 protein processing is uniquely regulated during meiosis.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Meiotic recombination is essential for accurate chromosome segregation.
  • The Dmc1 recombinase and Mei5-Sae3 complex are key players in meiotic DNA repair.
  • The precise mechanism by which Mei5-Sae3 facilitates Dmc1 assembly is not fully understood.

Purpose of the Study:

  • To investigate the role of basic residues in the Mei5 protein for Dmc1 assembly.
  • To elucidate the function of the Mei5-Sae3 complex in homology-mediated DNA repair during meiosis.
  • To explore the regulation of Mei5-Sae3 function by protein processing.

Main Methods:

  • Creation and characterization of mei5 mutants with specific amino acid substitutions.
  • Analysis of Mei5-Sae3 complex formation and Dmc1 assembly in yeast.
  • Investigation of Mei5 protein stability and localization in meiotic and mitotic cells.

Main Results:

  • The conserved residue Arg97 in Mei5 is critical for Mei5-Sae3 complex formation and Dmc1 assembly.
  • Mutations at Arg117 or Lys133 lead to a C-terminally truncated Mei5 protein during meiosis.
  • The truncated Mei5-R117A protein is specifically detected in meiotic cells, not mitotic cells.

Conclusions:

  • Arg97 of Mei5 is essential for its interaction with Sae3 and subsequent Dmc1 assembly.
  • Meiosis-specific posttranslational processing of Mei5 regulates its function in Dmc1-mediated recombination.
  • These findings highlight a unique regulatory mechanism controlling DNA repair during yeast meiosis.
Keywords:
Dmc1Mei5‐Sae3Rad51cleavagemeiotic recombination

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