Related Experiment Video
Updated: Jun 13, 2026
09:23
Lateral Root Inducible System in Arabidopsis and Maize
Published on: January 14, 2016
13.8K
A Small Auxin-Up RNA Gene, IbSAUR36, Regulates Adventitious Root Development in Transgenic Sweet Potato
Yuanyuan Zhou1, Aixian Li1, Taifeng Du1
1Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Genes
|June 27, 2024
Summary
The IbSAUR36 gene promotes sweet potato adventitious root development by enhancing auxin signaling and reducing lignin and JA pathways. This finding offers insights into regulating root growth in crops.
Area of Science:
- Plant Molecular Biology
- Plant Physiology
- Genetics
Background:
- Small auxin-upregulated RNAs (SAURs) are key regulators of plant growth and development.
- Limited knowledge exists on SAUR gene functions in sweet potato root development.
Purpose of the Study:
- To clone and functionally analyze the IbSAUR36 gene in sweet potato.
- To investigate the role of IbSAUR36 in adventitious root development.
Main Methods:
- Gene cloning and protein localization.
- Quantitative gene expression analysis under hormone treatments.
- Promoter analysis and GUS reporter assays in Arabidopsis.
- Agrobacterium rhizogenes-mediated transformation for generating transgenic sweet potato roots.
Main Results:
- IbSAUR36 protein localized to the nucleus and plasma membrane.
- Gene expression was high in pencil roots and leaves, induced by IAA, and downregulated by MeJA.
- IbSAUR36 showed high expression in young tissues of Arabidopsis.
- Overexpression of IbSAUR36 promoted IAA accumulation and signaling, while downregulating lignin and JA signaling pathways.
Conclusions:
- IbSAUR36 plays a crucial role in adventitious root development in sweet potato.
- The gene regulates root development by modulating auxin signaling, lignin synthesis, and JA signaling pathways.
More Related Videos
08:31Agrobacterium tumefaciens and Agrobacterium rhizogenes-Mediated Transformation of Potato and the Promoter Activity of a Suberin Gene by GUS Staining
Published on: March 29, 2019
29.5K
04:09Author Spotlight: Streamlining Composite Plant Production via Agrobacterium rhizogenes-Mediated Hairy Root Transformation
Published on: June 30, 2023
2.1K
Related Concept Videos
01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
02:23RNA Editing
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
02:28Leaky Scanning
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
02:30siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
02:57piRNA - Piwi-interacting RNAs
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
02:15Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...