Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Allergic Reactions02:06

Allergic Reactions

27.3K
Overview
27.3K
Cross-reactivity00:42

Cross-reactivity

31.0K
Overview
31.0K
Hypersensitivities01:30

Hypersensitivities

580
Hypersensitivity, also known as a hypersensitivity reaction or allergic reaction, is a condition where the body's immune system reacts abnormally to a foreign substance. Such substances, that cause hypersensitivity are referred to as an allergen, could be something typically harmless to most people, like pollen or certain foods.
Types of Hypersensitivities
Hypersensitivity reactions are categorized into four types: Type 1, Type 2, Type 3, and Type 4. Each type has a distinct mechanism...
580
Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

483
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
483

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondrial Adaptations to Exercise Training in Equine Skeletal Muscle: A Narrative Review.

Life (Basel, Switzerland)·2026
Same author

Involution of IgE sensitization to house dust mite allergen molecules by age.

Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology·2026
Same author

Combined Exposure to Ragweed and House Dust Mite Exacerbates Airway Epithelial Barrier Dysfunction: A Multimodal Approach.

Medicina (Kaunas, Lithuania)·2026
Same author

Irritant occupational exposure and specific-IgE sensitisation in the EGEA cohort.

Occupational and environmental medicine·2026
Same author

Discovery of tumor antigens in renal cell carcinoma and prospects for clinical application.

Frontiers in immunology·2026
Same author

Birch Pollen Exposure Boosts Mal d 1-Specific IgE and Sensitivity to Apple.

Allergy·2026

Related Experiment Video

Updated: Jun 22, 2025

Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens
09:09

Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens

Published on: February 24, 2021

3.0K

Non-Specific Lipid Transfer Protein Amb a 6 Is a Source-Specific Important Allergenic Molecule in Ragweed Pollen.

Manuela Grijincu1,2, Gabriela Tănasie1,2, Lauriana-Eunice Zbîrcea1,2

  • 1Center of Immuno-Physiology and Biotechnologies, Department of Functional Sciences, Victor Babeș University of Medicine and Pharmacy, 300041 Timișoara, Romania.

International Journal of Molecular Sciences
|June 27, 2024
PubMed
Summary

Ragweed pollen allergy affects millions globally. This study produced and characterized recombinant Amb a 6, a key ragweed allergen, revealing its significant role in respiratory allergies and potential for improved diagnostics and immunotherapy.

Keywords:
Amb a 6ImmunoCAPallergybasophil activation testcommon ragweednsLTPrecombinant allergen

More Related Videos

Humanized Mediator Release Assay as a Read-Out for Allergen Potency
10:22

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

3.5K
Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10
10:42

Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10

Published on: November 19, 2016

7.6K

Related Experiment Videos

Last Updated: Jun 22, 2025

Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens
09:09

Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens

Published on: February 24, 2021

3.0K
Humanized Mediator Release Assay as a Read-Out for Allergen Potency
10:22

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

3.5K
Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10
10:42

Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10

Published on: November 19, 2016

7.6K

Area of Science:

  • Immunology
  • Allergology
  • Biochemistry

Background:

  • Common ragweed pollen is a major global allergen, causing widespread respiratory allergies like rhinitis and asthma.
  • Non-specific lipid transfer proteins (nsLTPs) are stable allergens found in pollen and food, capable of inducing severe allergic reactions.
  • Amb a 6 is an nsLTP allergen identified in common ragweed pollen.

Purpose of the Study:

  • To produce recombinant, structurally folded Amb a 6 (rAmb a 6) protein.
  • To characterize the physicochemical and immunological properties of rAmb a 6.
  • To evaluate the allergenic potential and clinical relevance of rAmb a 6 in ragweed-allergic patients.

Main Methods:

  • Recombinant rAmb a 6 was expressed in *Spodoptera frugiperda* Sf9 insect cells.
  • Physicochemical characterization using mass spectrometry and circular dichroism (CD) spectroscopy.
  • Immunological evaluation through IgE-binding assays with patient sera and basophil activation tests.

Main Results:

  • Pure rAmb a 6 was successfully produced with a molecular weight of 10,963 Da and confirmed fold.
  • rAmb a 6 is a distinct nsLTP allergen, recognized by 30% of ragweed-allergic patients, with IgE reactivity linked to respiratory symptoms.
  • rAmb a 6 demonstrated significant allergenic activity, with some patients showing higher IgE levels to rAmb a 6 than to the major ragweed allergen Amb a 1.

Conclusions:

  • Amb a 6 is a significant, source-specific ragweed pollen allergen.
  • rAmb a 6 exhibits potent allergenic activity and is recognized by a substantial portion of allergic individuals.
  • Amb a 6 should be considered for improved diagnosis and allergen-specific immunotherapy strategies for ragweed pollen allergy.