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Updated: Jun 22, 2025
Detection of Functional Matrix Metalloproteinases by Zymography
Published on: November 8, 2010
Determination of Matrix Metalloproteinase 2 in Biological Samples Using a 3D Stochastic Microsensor Based on Graphene
Catalina Cioates Negut1, Ruxandra-Maria Ilie-Mihai1, Raluca-Ioana Stefan-van Staden1
1Laboratory of Electrochemistry and PATLAB, National Institute for Research and Development in Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021 Bucharest, Romania.
Abstract:
The levels of the MMPs in the biological samples of confirmed patients with gastric cancer are significantly elevated compared to those found in healthy people. Therefore, a novel 3D stochastic microsensor based on graphene oxide, modified with gold nanoparticles and (Z)-N-(pyridin-4-yl-methyl) octadec-9-enamide (namely N2-AuNP/GO), was designed for the determination of MMP-2 in biological samples, and validated for the screening tests of biological samples in order to be used for the early diagnosis of gastric cancer. The proposed sensor presents a low limit of quantification (1.00 × 10-22 g mL-1), high sensitivity (1.84 × 107 s-1 g-1 mL), and a wide working concentration range (1.00 × 10-22-1.00 × 10-7 g mL-1). Recovery values higher than 99.15% were recorded for the assay of MMP-2 in whole blood, gastric tissue tumors, saliva, and urine samples.
