Triaglycerol metabolism in Saccharomyces cerevisiae. Relation to phospholipid synthesis

The acylglycerol content of Saccharomyces cerevisiae has been examined during cellular growth. The cells maintained a constant amount of phospholipid and diacylglycerol throughout growth. Triacylglycerol content fell in the early exponential phase of growth and then increased sharply upon entry of the culture into the stationary growth phase. Pulse-chase experiments with [1-14C]oleic acid and [2-3H]- and [1-14C]glycerol indicated that the triacylglycerol molecule was utilized for phospholipid synthesis in early exponential phase probably through a diacylglycerol intermediate. A substantial turnover of phospholipid during growth was also apparent. No role for the triacylglycerol could be found in regulating the fatty acid species of the phospholipid nor in the storage of fatty acid for energy metabolism.