Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deep learning-enabled discovery of antibiotics effective against <i>Neisseria gonorrhoeae</i>.

Science translational medicine·2026
Same author

Pneumococcal membrane particles promote serotype-independent cellular and humoral immunity and protect against pneumococcal colonization.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Metabolic assessment of iPSC-derived neurons under ketone-enriched condition: Ketone sensor development and BHB-driven metabolic adaptation.

iScience·2026
Same author

Microfluidic toolbox using padlock probes and rolling circle amplification for direct detection and genotyping of viral RNA.

RSC advances·2026
Same author

Mitochondrial CircRNA CircMT-RNR2 Safeguards Antioxidant Defense to Support Fibroblast Functions in Wound Repair.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Exposing the hidden: establishing immunity to Plasmodium liver stage infection.

Trends in parasitology·2025

Related Experiment Video

Updated: Jun 16, 2026

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
04:17

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

732

An Automated Versatile Diagnostic Workflow for Infectious Disease Detection in Low-Resource Settings.

Miren Urrutia Iturritza1,2, Phuthumani Mlotshwa1, Jesper Gantelius1,2

  • 1Department of Global Public Health, Karolinska Institutet, 17177 Stockholm, Sweden.

Micromachines
|June 27, 2024
PubMed
Summary

This study presents a versatile, low-cost diagnostic workflow using open-source automation for infectious diseases. Automation of molecular tests, like for *Neisseria meningitidis*, proved faster and cheaper than manual methods.

Keywords:
infectious diseasesmicroarraymodular automationopen-sourcerecombinase polymerase amplificationsignal enhancement

More Related Videos

Remote Laboratory Management: Respiratory Virus Diagnostics
14:56

Remote Laboratory Management: Respiratory Virus Diagnostics

Published on: April 6, 2019

33.1K
One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
08:30

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures

Published on: July 9, 2012

25.5K

Related Experiment Videos

Last Updated: Jun 16, 2026

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning
04:17

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

732
Remote Laboratory Management: Respiratory Virus Diagnostics
14:56

Remote Laboratory Management: Respiratory Virus Diagnostics

Published on: April 6, 2019

33.1K
One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
08:30

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures

Published on: July 9, 2012

25.5K

Area of Science:

  • Biotechnology
  • Molecular Diagnostics
  • Laboratory Automation

Background:

  • Laboratory automation enhances sample analysis throughput and reduces errors.
  • Automating diagnostic testing in near-patient settings offers rapid results and minimizes contamination risks.
  • Existing automation platforms are often costly and lack adaptability for new protocols.

Purpose of the Study:

  • To develop a versatile, user-friendly, rapid, and reliable diagnostic testing workflow.
  • To combine open-source modular automation with adaptable molecular biology protocols for infectious disease diagnosis.
  • To demonstrate the feasibility of automating paper-based diagnostics using open-source platforms.

Main Methods:

  • Integration of open-source modular automation (Opentrons) with automation-compatible molecular biology protocols.
  • Development of a low-cost diagnostic test for *Neisseria meningitidis*.
  • Utilizing magnetic beads for DNA isolation, isothermal amplification, and paper-based microarray detection.

Main Results:

  • Demonstrated the feasibility of automating the diagnostic workflow.
  • The automated method for *Neisseria meningitidis* detection was faster and cheaper than manual processing.
  • The integrated system enables versatile infectious disease diagnostics.

Conclusions:

  • Open-source modular automation combined with adaptable molecular protocols provides a versatile solution for infectious disease diagnostics.
  • This approach offers a cost-effective and efficient alternative to traditional manual diagnostic methods.
  • The demonstrated low-cost *N. meningitidis* test on paper-based microarrays highlights the potential of this integrated workflow.