Simvastatin-Encapsulated Topical Liposomal Gel for Augmented Wound Healing: Optimization Using the Box-Behnken Model,...

Area of Science:

• Pharmaceutical Sciences
• Biomedical Engineering

Background:

• Statins, known for cholesterol regulation, also promote wound healing when given systemically.
• Topical application of statins for wound healing remains underexplored.

Purpose of the Study:

• To formulate, evaluate, and optimize Simvastatin (SIM)-encapsulated liposome gel for topical wound healing.
• To assess the wound healing efficacy of the optimized liposomal gel formulation.

Main Methods:

• Liposomes encapsulating SIM were formulated using the thin-film hydration method.
• Response surface methodology (RSM) optimized formulation variables (DOTAP, Span 80, cholesterol).
• Characterization included zeta potential, entrapment efficacy, particle size, and rheological properties.

Main Results:

• Optimized formulation (F-07) showed high stability (16.56 mV zeta potential) and SIM encapsulation (95.6%).
• Particle size (190.3 nm) confirmed structural integrity; pseudoplastic flow ensured ease of application and enhanced penetration.
• The liposomal gel demonstrated prolonged SIM release and significant wound size reduction in mice by day 16.

Conclusions:

• Liposomal gels are a promising drug delivery strategy for topical Simvastatin (SIM).
• This approach enhances SIM's effectiveness in promoting wound healing.