Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Folding01:25

Protein Folding

7.9K
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
7.9K
Factors Affecting Dissolution: Drug Permeability, Stability and Stereochemistry01:20

Factors Affecting Dissolution: Drug Permeability, Stability and Stereochemistry

196
Orally administered drugs primarily enter the systemic circulation via passive diffusion through the intestinal membranes. The drug's absorption is influenced by drug stability in the gastrointestinal GI tract, membrane permeability, the surface area available for absorption, luminal drug concentration, and residence time in the lumen. Drug permeability can be enhanced by adjusting the lipophilicity, polarity, or molecular size of the drug, promoting its passive transport across intestinal...
196
Protein Organization01:24

Protein Organization

6.4K
Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence....
6.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Capillary flow-driven paper-based microfluidic sensor for NDMA detection in water.

Lab on a chip·2026
Same author

Integrated Magnetophoretic-Electrochemical platforms for portable detection of HER2 in breast cancer diagnosis.

Biosensors & bioelectronics·2026
Same author

Smartphone-Based Peptide Nucleic Acid (PNA) Probe-Assisted Potentiometric Biosensor for Point-of-Care Testing of SARS-CoV-2 Nucleic Acid.

Analytical chemistry·2026
Same author

Detection of Aerosolized Protein Using a Condensation Growth Tube Coupled with an Electrochemical Immunoassay on Screen-Printed Carbon Electrodes.

Analytical chemistry·2026
Same author

Capillary-Driven Duplex Microfluidic Device Enabling Simultaneous Electrochemical Immunoassays for Detecting SARS-CoV-2 and Respiratory Syncytial Virus.

ACS sensors·2026
Same author

Sponge-Based Flow Control in Laminate Capillary-Driven Electrochemical Microfluidic Devices for Viscous Sample Analysis.

Analytical chemistry·2026

Related Experiment Video

Updated: Jun 22, 2025

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
11:14

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

Published on: April 14, 2015

16.1K

Stability of Protein Pharmaceuticals: Recent Advances.

Mark Cornell Manning1,2, Ryan E Holcomb3,4, Robert W Payne3,4

  • 1Legacy BioDesign LLC, Johnstown, CO, USA. manning@legacybiodesign.com.

Pharmaceutical Research
|June 27, 2024
PubMed
Summary

Recent advances enhance understanding of liquid protein formulation and stabilization. New strategies address complex challenges like high-concentration protein solutions and co-formulations for therapeutic proteins.

Keywords:
co-formulationformulationmonoclonal antibodiesprotein stabilityproteins

More Related Videos

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries
10:58

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Published on: September 6, 2012

10.4K
How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
07:22

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Published on: February 11, 2019

28.2K

Related Experiment Videos

Last Updated: Jun 22, 2025

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
11:14

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

Published on: April 14, 2015

16.1K
Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries
10:58

Combinatorial Synthesis of and High-throughput Protein Release from Polymer Film and Nanoparticle Libraries

Published on: September 6, 2012

10.4K
How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
07:22

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Published on: February 11, 2019

28.2K

Area of Science:

  • Biochemistry
  • Pharmaceutical Sciences
  • Protein Chemistry

Background:

  • Significant progress has been made in liquid protein formulation and stabilization.
  • Mechanistic understanding of protein-excipient interactions has improved, enabling rational formulation development.
  • The field is advancing towards more complex formulations, including high-concentration solutions for subcutaneous delivery and co-formulations.

Purpose of the Study:

  • To review recent advances in the formulation and stabilization of therapeutic proteins in the liquid state.
  • To discuss the mechanistic understanding of protein-excipient interactions.
  • To explore challenges and strategies for complex formulations, particularly high-concentration protein solutions.

Main Methods:

  • Review of recent scientific literature on protein formulation and stability.
  • Discussion of chemical degradation pathways and physical instability issues.
  • Analysis of protein-interface interactions, predictive stability methods, and chemical-physical instability interplay.

Main Results:

  • Enhanced understanding of protein degradation and instability mechanisms.
  • Development of more rational approaches to protein formulation.
  • Insights into strategies for high-concentration and co-formulations of therapeutic proteins.
  • While monoclonal antibodies (mAbs) are often studied, principles apply broadly to therapeutic proteins.

Conclusions:

  • Advances in understanding protein-excipient interactions facilitate rational formulation design.
  • Current research addresses complex formulation challenges like high protein concentrations and co-formulations.
  • Knowledge of chemical and physical instability is crucial for developing stable liquid protein therapeutics.