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Updated: Aug 9, 2026

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Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
Published on: April 11, 2016
[Use of nucleic acid preparative electrophoresis in molecular oncological research]
Abstract:
Application of preparative electrophoresis in agarose gel for isolation of chromosomal and extrachromosomal genetic elements is described. The above-mentioned method is used for fractionation of chromosomal DNA according to the molecular weight; for isolation of the plasmid containing the insertion of the viral oncogene myc and for separation of the viral oncogene myc insertion from the vector (a linear form of plasmid pBR 322). The method can be successfully applied in molecular biology, microbiology and medicine.

