Lysozyme-sensitive plasmonic hydrogel nanocomposite for colorimetric dry-eye inflammation biosensing

Area of Science:

• Biomaterials Science
• Nanotechnology
• Ophthalmology

Background:

• Lysozyme detection in ocular fluids is vital for diagnosing eye conditions such as dry-eye syndrome.
• Hydrogel nanocomposites offer a promising platform for rapid and accurate biomolecule sensing.

Purpose of the Study:

• To develop a sensitive, easy-to-use biosensor for lysozyme detection in ocular fluids.
• To create a colorimetric platform for non-invasive monitoring of eye health.

Main Methods:

• Fabrication of a biosensor combining hydrogel, electrospun nanofibers (PLCL), and plasmonic nanoparticles (AgNPls).
• Utilizing localized surface plasmon resonance (LSPR) for quantitative detection.
• Characterization of platform layers and investigation of lysozyme detection using colorimetry and UV-Vis spectroscopy.

Main Results:

• The developed biosensor demonstrated high sensitivity and rapid response time for lysozyme detection.
• The platform exhibited a colorimetric response, enabling naked-eye detection.
• Lysozyme detection was validated at concentrations relevant to dry-eye syndrome and other ocular diseases.

Conclusions:

• The novel hydrogel-based biosensor is a promising tool for early diagnosis and monitoring of eye diseases.
• The sensor's sensitivity, rapid response, and ease of use facilitate non-invasive ocular diagnostics.
• This technology holds potential for improved patient outcomes in ophthalmology.