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Updated: Jul 19, 2026

An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
Published on: September 15, 2015
Metaproteomic Analysis of Biogas Plants: A Complete Workflow from Lab to Bioinformatics
Robert Heyer1,2, Kay Schallert3,4, Marie Briza5
1Leibniz Institute for Analytical Sciences - ISAS, Multidimensional Omics Analysis Group, Dortmund, Germany. robert.heyer@isas.de.
Abstract:
Metaproteomics represents a promising and fast method to analyze the taxonomic and functional composition of biogas plant microbiomes. However, metaproteomics sample preparation and bioinformatics analysis is still challenging due to the sample complexity and contaminants. In this chapter, a tailored workflow including sampling, phenol extraction in a ball mill, amido black protein quantification, FASP digestion, LC-MS/MS measurement as well as bioinformatics and biostatistical data evaluation are here described for the metaproteomics advancements applied to biogas plant samples.
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