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Optical Label-Free Aptasensor Based on Weak Value Amplification for Real-Time and Ultrasensitive Detection of IgE
Bei Wang1,2, Lingqin Meng1,2, Gengyu Liang1,2
1Institute of Optical Imaging and Sensing, Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China.
ACS Sensors
|June 29, 2024
Summary
This study introduces a novel aptasensor using weak value amplification (WVA) for sensitive, real-time detection of immunoglobulin E (IgE) in serum. This method enables early allergy diagnosis with high accuracy and low cost.
Area of Science:
- Biomedical Engineering
- Biosensing Technology
- Immunology
Background:
- Allergy is a widespread condition with a growing population affected by industrialization and lifestyle changes.
- Serum immunoglobulin E (IgE) levels are key indicators for diagnosing allergies.
- Existing diagnostic methods may lack sensitivity or real-time monitoring capabilities.
Purpose of the Study:
- To develop a simple, low-cost, label-free aptasensor for real-time, quantitative detection of IgE in serum.
- To enable early and accurate diagnosis of allergic and hypersensitive conditions.
- To demonstrate the feasibility of the sensing strategy for complex biological samples.
Main Methods:
- A novel aptasensor strategy combining weak value amplification (WVA) with an imaging weak measurement system.
- Anti-IgE aptamers immobilized on a dopamine-modified prism surface to selectively capture IgE.
- Detection based on changes in surface refractive index, visualized as alterations in light intensity.
Main Results:
- Achieved a wide detection range of 0.01 ng/mL to 2 μg/mL for IgE in buffer.
- Demonstrated a high resolution as low as 4.3 pg/mL.
- Successfully validated the method in human serum samples, showing specific IgE recognition without interference from other proteins.
Conclusions:
- The proposed WVA-aptasensing strategy offers a highly sensitive and specific method for IgE detection.
- This approach facilitates early and accurate diagnosis of allergies.
- The sensing strategy shows potential for broader applications in detecting other relevant biomolecules.
