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Weak Value Amplification Based Optical Sensor for High Throughput Real-Time Immunoassay of SARS-CoV-2 Spike Protein
Xiaonan Zhang1,2, Lizhong Zhang1, Han Li1
1Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China.
Biosensors
|July 26, 2024
Summary
This study introduces a novel weak measurement sensor for high-throughput immunoassays. The developed chip enables real-time analysis of SARS-CoV-2 spike protein with a low detection limit.
Area of Science:
- Quantum Measurement
- Biomedical Engineering
- Analytical Chemistry
Background:
- Accurate and efficient immunoassays are crucial for high-throughput analysis.
- Existing methods may lack precision or scalability for rapid diagnostics.
Purpose of the Study:
- To develop a novel, high-throughput immunoassay method using a weak measurement interface sensor.
- To enable real-time monitoring of molecular binding, specifically for SARS-CoV-2 spike protein detection.
Main Methods:
- Utilized a weak measurement technique with pre- and post-selection states to amplify signals.
- Developed a six-flow-channel chip for simultaneous immunoassays.
- Performed real-time image analysis of flow channels to determine relative intensity and binding characteristics.
Main Results:
- Achieved a limit of detection (LOD) of 0.85 ng/mL for SARS-CoV-2 spike protein.
- Demonstrated a high correlation (R²=0.91) between spike protein concentration and relative intensity using Log-Log fitting.
- Verified the method's ability to monitor molecular binding and differentiate binding capacities.
Conclusions:
- The weak measurement interface sensor offers a simple, precise, and high-throughput solution for immunoassays.
- This method provides a sensitive platform for detecting SARS-CoV-2 spike protein and analyzing molecular interactions.
