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Rohon-beard neurons do not succumb to programmed cell death during zebrafish development.

Kendra E Liu1, Sarah Kucenas2

  • 1Neuroscience Graduate Program, University of Virginia, Charlottesville, VA, 22904, USA; Program in Fundamental Neuroscience, University of Virginia, Charlottesville, VA, 22904, USA.

Developmental Biology
|June 29, 2024
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Summary
This summary is machine-generated.

Early mechanosensory Rohon-Beard neurons (RBNs) in zebrafish do not undergo programmed cell death as previously thought. Instead, these neurogenin1-positive RBNs persist and change morphology during early neural development.

Keywords:
ApoptosisNeural developmentProgrammed cell deathRohon-Beard neuronZebrafish

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Neural development involves circuit refinement through cell death and synaptic pruning.
  • Rohon-Beard neurons (RBNs) are the earliest mechanosensory neurons, crucial for rudimentary motor circuits.
  • RBNs were previously thought to undergo significant cell death as dorsal root ganglion (DRG) neurons develop.

Purpose of the Study:

  • To investigate the fate of neurogenin1-positive (ngn1+) RBNs during early zebrafish development.
  • To challenge the established understanding of RBN programmed cell death.
  • To explore the persistence and potential functional changes of RBNs.

Main Methods:

  • Utilized zebrafish as a model organism.
  • Employed genetic labeling (neurogenin1-positive) to track RBNs.
  • Observed RBNs from early development up to 15 days post fertilization (dpf).

Main Results:

  • Contrary to previous studies, ngn1+ RBNs do not undergo widespread programmed cell death.
  • RBNs persist in zebrafish until at least 15 dpf.
  • Observed medialization and shrinkage of RBN somas starting at 2 dpf, with gradual ngn1 downregulation.

Conclusions:

  • The widespread programmed cell death of RBNs is not a fundamental aspect of early zebrafish neural development.
  • ngn1+ RBNs persist and undergo significant morphological and molecular changes.
  • This finding necessitates a re-evaluation of RBN function, persistence, and circuit refinement mechanisms.