Decoding dysregulated genes, molecular pathways and microRNAs involved in cervical cancer

Area of Science:

• Oncology
• Genomics
• Molecular Biology

Background:

• Human papillomavirus (HPV) is a major cause of cervical cancer.
• Understanding the molecular mechanisms of HPV-associated cervical cancer is crucial for developing effective treatments.
• Previous research has focused on identifying key genes and pathways involved in cervical carcinogenesis.

Purpose of the Study:

• To identify dysregulated genes, molecular pathways, and regulatory mechanisms in HPV-associated cervical cancers.
• To investigate disease-associated genes, Gene Ontology, survival prognosis, transcription factors, and microRNAs (miRNAs) in cervical carcinogenesis.
• To enhance the comprehension of HPV-linked cervical cancer.

Main Methods:

• Utilized 10 Gene Expression Omnibus (GEO) datasets for gene expression analysis in cervical cancer.
• Employed GEO2R for identifying differentially expressed genes (DEGs) between cancer and healthy tissues.
• Applied bioinformatic techniques for pathway and functional enrichment analysis, including investigation of HPV infection links.

Main Results:

• Identified 48 DEGs in cervical cancer tissues compared to healthy controls.
• CCND1, CCNA2, and SPP1 were identified as key dysregulated genes in HPV-associated cervical cancer.
• Five common miRNAs (miR-7-5p, miR-16-5p, miR-124-3p, miR-10b-5p, miR-27a-3p) were found to target these genes, with SP1 identified as a common transcription factor for hsa-miR-27a-3p targets.

Conclusions:

• Identified DEGs, molecular pathways, and transcription factors critical for HPV-associated cervical cancer progression.
• Enhanced understanding of cervical cancer pathogenesis.
• Provided potential therapeutic and intervention targets for cervical cancer treatment.