[Introduction of a decalcification method for bone marrow biopsy tissue]

Area of Science:

• Hematopathology
• Histotechnology

Background:

• Bone marrow biopsy is vital for diagnosing lymphohematopoietic diseases.
• Decalcification is a critical pre-treatment step, but balancing efficiency with antigen preservation is challenging.

Purpose of the Study:

• To introduce an efficient and antigen-preserving decalcification method for bone marrow biopsy specimens.
• To address the limitations of current decalcification techniques that can lead to difficult sectioning or antigen damage.

Main Methods:

• Utilized a decalcification solution containing 12% formic acid and 8% hydrochloric acid.
• Performed decalcification at room temperature with agitation using a shaker.

Main Results:

• Achieved high decalcification efficiency.
• Significantly reduced antigen loss compared to conventional methods.
• Facilitated easier tissue sectioning and staining.

Conclusions:

• The described method offers an effective approach for bone marrow biopsy decalcification.
• This technique supports accurate morphological and molecular diagnostic testing by preserving tissue integrity and antigenicity.