Chitinophaga pollutisoli sp. nov., isolated from contaminated sediment

A Gram-stain-negative, yellow-pigmented, and facultatively aerobic bacterium, designated strain GPA1^T, was isolated from plastic waste landfill soil in the Republic of Korea. The cells were non-motile short rods exhibiting oxidase-negative and catalase-positive activities. Growth was observed at 15-40 °C (optimum, 30 °C), at pH 6.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 0-2.5 % (w/v) NaCl (optimum, 0 %). Menaquinone-7 was the sole respiratory quinone, and iso-C_15 : 0, C_16 : 1  ω5c, and iso-C_17 : 0 3-OH were the major cellular fatty acids (>10 % of the total fatty acids). Phosphatidylethanolamine was identified as a major polar lipid. Phylogenetic analyses based on 16S rRNA gene sequences and 120 concatenated marker protein sequences revealed that strain GPA1^T formed a distinct lineage within the genus Chitinophaga. The genome of strain GPA1^T was 6078 kb in size with 53.8 mol% G+C content. Strain GPA1^T exhibited the highest similarity to Chitinophaga rhizosphaerae T16R-86^T, with a 98.6 % 16S rRNA gene sequence similarity, but their average nucleotide identity and digital DNA-DNA hybridization values were 82.5 and 25.9 %, respectively. Based on its phenotypic, chemotaxonomic, and phylogenetic characteristics, strain GPA1^T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga pollutisoli sp. nov. is proposed. The type strain is GPA1^T (=KACC 23415^T=JCM 36644^T).