Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Computerized analysis of enzyme cascade reactions using continuous rate data obtained with an ELISA reader.

S D Carson

    Computer Programs in Biomedicine
    |January 1, 1985
    PubMed
    Summary

    New software analyzes enzyme-cascade assays by measuring chromophore acceleration rates. This enables simultaneous kinetic experiments, enhancing enzyme reaction analysis with spectrophotometry.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cadmium causes vesicle leakage under conditions which favor reconstitution of tissue factor-vesicle complexes.

    The Journal of membrane biology·2015
    Same author

    The coxsackievirus and adenovirus receptor.

    Current topics in microbiology and immunology·2008
    Same author

    The effect of an imprecise map on interval mapping QTLs.

    Genetical research·2005
    Same author

    QTL associations for density and diameter in Pinus radiata and the potential for marker-aided selection.

    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2003
    Same author

    Toward testing the hypothesis that group B coxsackieviruses (CVB) trigger insulin-dependent diabetes: inoculating nonobese diabetic mice with CVB markedly lowers diabetes incidence.

    Journal of virology·2002
    Same author

    Coxsackievirus and adenovirus receptor (CAR) binds immunoglobulins.

    Biochemistry·2001

    Area of Science:

    • Biochemistry
    • Enzymology
    • Analytical Chemistry

    Background:

    • Enzyme-cascade assays are crucial for studying complex biological reactions.
    • Accurate kinetic analysis is essential for understanding enzyme mechanisms and rates.
    • Existing methods may limit the simultaneous analysis of multiple kinetic experiments.

    Purpose of the Study:

    • To develop and present novel software for analyzing continuous-rate enzyme-cascade assays.
    • To enable the precise measurement of initial reaction rates within enzyme cascades.
    • To facilitate the simultaneous analysis of multiple kinetic experiments.

    Main Methods:

    • Utilized an ELISA spectrophotometer and a synthetic chromogenic substrate for assays.
    • Developed two software programs for data analysis.

    Related Experiment Videos

  • Program 1: Linear regression to analyze reaction curves (rate of acceleration vs. square of time).
  • Program 2: Simplex algorithm to fit rate data to the Hill equation for standard curve determination.

    • Main Results:

    • The software accurately measures the rate of chromophore acceleration, reflecting initial reaction rates.
    • Linear regression effectively determines acceleration rates from reaction curves.
    • The Simplex algorithm successfully establishes assay standard curves using the Hill equation.
    • The programs allow for the analysis of multiple enzyme-cascade assays concurrently.

    Conclusions:

    • The developed software provides a robust method for analyzing enzyme-cascade kinetics.
    • Simultaneous analysis of multiple assays is feasible, improving experimental efficiency.
    • This approach enhances the study of enzyme reaction rates and mechanisms.