Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

4.0K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
4.0K
Photoluminescence: Applications01:14

Photoluminescence: Applications

386
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
386

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diagnostic performance and interchangeability of two commercial anti-RSV-IgG and IgA ELISAs across clinically relevant populations.

Microbiology spectrum·2026
Same author

Rare twin cysteine residues in the HIV-1 envelope variable region 1 link to neutralization escape and breadth development.

Cell host & microbe·2026
Same author

Low-positive CMV IgG serostatus before allogeneic hematopoietic cell transplantation is not associated with increased CMV reactivation risk.

Bone marrow transplantation·2026
Same author

Detecting <i>erm</i>-Mediated Inducible Macrolide-Lincosamide-Streptogramin B Resistance in Anaerobic Clinical Isolates.

Antibiotics (Basel, Switzerland)·2026
Same author

An integrated <i>i</i> <i>n vitro</i> platform and biophysical modeling approach for studying synaptic transmission in isolated neuronal pairs.

iScience·2026
Same author

Diagnostic Value and Outcomes of Systematic SARS-CoV-2 Screening in Asymptomatic Patients.

JAMA network open·2026
Same journal

Four-in-one multifunctional CoCu-NC@AuPt nanozyme integrated M13 phage-displayed nanobody based multimodal lateral flow immunoassay for bovine lactoferrin detection.

Biosensors & bioelectronics·2026
Same journal

A novel capillary-driven dual-mode imaging flow cytometry system for malaria parasite detection and quantification.

Biosensors & bioelectronics·2026
Same journal

Liver-targeted alkaline phosphatase-activatable fluorescent probe for imaging liver fibrosis and screening anti-fibrotic natural products.

Biosensors & bioelectronics·2026
Same journal

GLASS-seq: a gel-anchored, ligation-assisted, scalable biosensing platform for low-cost regional spatial transcriptomics.

Biosensors & bioelectronics·2026
Same journal

CRISPR/Cas12a-based dual-modal signal platform using MIL-101(Fe) for colorimetric and electron spin resonance detection of HPV-16 nucleic acid.

Biosensors & bioelectronics·2026
Same journal

Fully automated centrifugal microfluidic system for self-calibrating isothermal nucleic acid quantification.

Biosensors & bioelectronics·2026
See all related articles

Related Experiment Video

Updated: Jun 21, 2025

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
10:10

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

7.9K

Iodide based electrochemical gold quantification method for lateral flow assays.

Yves Blickenstorfer1, Vlastimil Jirasko1, Alexander Tanno1

  • 1Laboratory of Biosensors and Bioelectronics, Institute of Biomedical Engineering, ETH Zurich, Zurich, Switzerland; Hemetron Ag, Thalwil, Switzerland.

Biosensors & Bioelectronics
|July 6, 2024
PubMed
Summary
This summary is machine-generated.

We developed a new electrochemical method for lateral flow assays (LFAs) that provides quantitative and sensitive results for at-home diagnostics. This breakthrough improves upon traditional yes/no tests, offering greater accuracy and lower detection limits.

More Related Videos

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

1.2K
Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

10.4K

Related Experiment Videos

Last Updated: Jun 21, 2025

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
10:10

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

7.9K
Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

1.2K
Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

10.4K

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Point-of-Care Diagnostics

Background:

  • Lateral flow assays (LFAs) offer convenient, cost-effective, and rapid at-home medical diagnostics.
  • Current LFAs are limited to qualitative (yes/no) results and lack the sensitivity of laboratory methods.
  • Existing optical readers for quantitative LFAs are often too expensive for widespread at-home use.

Purpose of the Study:

  • To develop a quantitative and sensitive readout method for LFAs.
  • To overcome the limitations of current qualitative LFAs and expensive optical readers.
  • To enable more advanced medical diagnostics at home.

Main Methods:

  • Developed an electrochemical readout procedure for LFAs.
  • Utilized voltage-triggered in-situ dissolution of gold nanoparticles (conventional LFA labels).
  • Quantified dissolved gold via electroplating and electrochemical measurement, achieving low-noise current signals.

Main Results:

  • Achieved superior detection limits compared to traditional optical techniques.
  • Demonstrated a quantitative and sensitive electrochemical readout for LFAs.
  • Developed hardware for the readout system, showing potential for low-cost electronics.

Conclusions:

  • The novel electrochemical readout significantly enhances LFA capabilities for at-home diagnostics.
  • This method offers improved sensitivity and quantitative results, surpassing optical limitations.
  • The technology is translatable to low-cost electronic devices, paving the way for accessible advanced diagnostics.