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Related Experiment Video

Updated: Jun 21, 2025

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling |

Published on: November 13, 2011

Transcriptomics : Approaches to Quantifying Gene Expression and Their Application to Studying the Human Brain.

Lora Liharska¹, Alexander Charney²

¹Icahn School of Medicine at Mount Sinai, New York, NY, USA. lora.liharska@icahn.mssm.edu.

Current Topics in Behavioral Neurosciences

|July 7, 2024

Summary

This chapter reviews transcriptomics, the study of gene expression. It covers evolving quantification methods, current technologies, and applications in human brain research within a multiomics context.

Keywords:

Data analysis Gene expression Multiomics NGS RNA RNA-seq Transcriptomics

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Area of Science:

Transcriptomics
Gene Expression Analysis
Neuroscience

Background:

The field of transcriptomics is rapidly evolving with new technologies.
Older gene expression quantification methods are frequently superseded.

Purpose of the Study:

To trace the historical development of gene expression quantification.
To provide an overview of the current state of transcriptomics.
To discuss transcriptomics applications in human brain studies and its role in multiomics.

Main Methods:

Historical review of transcriptomics methodologies.
Analysis of current transcriptomic technologies.
Integration of transcriptomics within multiomics research.

Main Results:

Documented evolution of gene expression measurement techniques.
Current landscape of transcriptomics tools and applications.
Positioning of transcriptomics in the broader multiomics field.

Conclusions:

Transcriptomics has undergone significant technological advancement.
Current transcriptomics offers powerful tools for biological research, particularly in neuroscience.
Transcriptomics is a key component of emerging multiomics approaches.