Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Recrystallization: Solid–Solution Equilibria01:10

Recrystallization: Solid–Solution Equilibria

1.1K
Recrystallization is a purification technique used to separate impurities from solid compounds. In this technique, no chemical reactions occur. Instead, it exploits physical properties only, specifically, the solubility differences between the desired compound and impurities, either at a single temperature or at different temperatures, and under other selected conditions. The solid-solution equilibrium (solubility equilibrium) of each component in the solution represents a binary phase...
1.1K
Crystal Growth: Principles of Crystallization01:25

Crystal Growth: Principles of Crystallization

1.8K
Crystallization is a phase transformation process in which crystals are precipitated from a supersaturated solution or formed from other sources. During crystallization, atoms or molecules arrange themselves into a well-defined, rigid crystal lattice to minimize energy.
Initiating crystallization involves manipulating the concentration of the solute and the temperature of the solution. Since crystal growth occurs when the ratio of concentration and solubility of the solute in the solvent...
1.8K
Colloidal precipitates01:09

Colloidal precipitates

546
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
546
Polymer Classification: Crystallinity01:21

Polymer Classification: Crystallinity

2.8K
Unlike ionic or small covalent molecules, polymers do not form crystalline solids due to the diffusion limitations of their long-chain structures. However, polymers contain microscopic crystalline domains separated by amorphous domains.
Crystalline domains are the regions where polymer chains are aligned in an orderly manner and held together in proximity by intermolecular forces. For example, chains in the crystalline domains of polyethylene and nylon are bound together by van der Waals...
2.8K
Factors Affecting Dissolution: Polymorphism, Amorphism and Pseudopolymorphism01:21

Factors Affecting Dissolution: Polymorphism, Amorphism and Pseudopolymorphism

295
Polymorphism refers to the existence of a drug substance in multiple crystalline forms, known as polymorphs. Recently, this term has been expanded to include solvates (forms containing a solvent), amorphous forms (non-crystalline forms), and desolvated solvates (forms from which the solvent has been removed).
Some polymorphic crystals possess lower aqueous solubility than their amorphous counterparts, leading to incomplete absorption. For instance, the oral suspension of Chloramphenicol, which...
295
Precipitation Processes01:12

Precipitation Processes

442
The experimental conditions in a gravimetric analysis should be optimized to maximize the particle size and purity of the obtained precipitate. Ideally, the concentration of the precipitating reagent should be low with effective stirring to maintain low relative supersaturation for the growth of large crystals. In homogeneous precipitation, the precipitant is slowly generated by a chemical reaction in the solution to avoid local reagent excesses. For example, urea decomposes gradually to...
442

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization and Monitoring of Isomalto/Malto-Polysaccharide Formation by Different 4,6-α-Glucanotransferases.

Journal of agricultural and food chemistry·2025
Same author

Investigation of κ-Carrageenan's Ice-Binding Properties Using Molecular Dynamics Simulation.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Screening of 14 Lactic Acid Bacteria for Fermentative Isomalto/Malto-Polysaccharide Synthesis.

Journal of agricultural and food chemistry·2025
Same author

Hard shell, soft blue-green core: Ecology, processes, and modern applications of calcification in terrestrial cyanobacteria.

iScience·2024
Same author

Capsular Exopolysaccharides from Two <i>Streptococcus thermophilus</i> Strains Differ in Their Moisture Sorption Behavior.

Foods (Basel, Switzerland)·2023
Same author

Influence of Arabinan Fine Structure, Galacturonan Backbone Length, and Degree of Esterification on the Emulsifying Properties of Acid-Extracted Sugar Beet Pectins.

Journal of agricultural and food chemistry·2023

Related Experiment Video

Updated: Jun 21, 2025

A Microfluidic Approach for the Study of Ice and Clathrate Hydrate Crystallization
08:01

A Microfluidic Approach for the Study of Ice and Clathrate Hydrate Crystallization

Published on: August 18, 2022

3.1K

Furcellaran: Impact of Concentration, Rheological Properties, and Structure on Ice Recrystallization Inhibition

Julian Gerhäuser1, Julia Hale2, Daniel Wefers3

  • 1Institute of Process Engineering in Life Sciences, Food Process Engineering, Karlsruhe Institute of Technology, Kaiserstraße 12, 76131 Karlsruhe, Germany.

Biomacromolecules
|July 8, 2024
PubMed
Summary

Furcellaran effectively inhibits ice crystal growth during frozen storage, similar to κ-carrageenan. However, its gel-like consistency at higher concentrations reduces this protective effect, indicating optimal use requires avoiding gel formation.

More Related Videos

Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification
09:43

Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification

Published on: May 5, 2017

8.7K
LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations
09:32

LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations

Published on: February 4, 2013

20.6K

Related Experiment Videos

Last Updated: Jun 21, 2025

A Microfluidic Approach for the Study of Ice and Clathrate Hydrate Crystallization
08:01

A Microfluidic Approach for the Study of Ice and Clathrate Hydrate Crystallization

Published on: August 18, 2022

3.1K
Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification
09:43

Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification

Published on: May 5, 2017

8.7K
LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations
09:32

LabVIEW-operated Novel Nanoliter Osmometer for Ice Binding Protein Investigations

Published on: February 4, 2013

20.6K

Area of Science:

  • Biophysics
  • Materials Science
  • Food Science

Background:

  • Recrystallization is a primary cause of damage in frozen biological materials.
  • Polysaccharides like κ-carrageenan are known to inhibit ice crystal growth.
  • Furcellaran, a related polysaccharide, was investigated for its cryoprotective properties.

Purpose of the Study:

  • To evaluate the concentration-dependent effect of furcellaran on ice crystal growth and recrystallization.
  • To characterize furcellaran's structure, sulfate content, and rheological properties.
  • To determine the optimal conditions for furcellaran's recrystallization inhibition activity.

Main Methods:

  • 1H nuclear magnetic resonance spectroscopy for structural analysis.
  • Ion chromatography and high-performance size-exclusion chromatography for sulfate content and molecular weight determination.
  • Rheological measurements to assess solution properties at varying furcellaran concentrations.

Main Results:

  • Furcellaran demonstrated comparable ice growth inhibition to κ-carrageenan.
  • Increasing furcellaran concentration led to a gel-like state at 5 g/L.
  • This gel formation correlated with reduced recrystallization inhibition and larger ice crystals.

Conclusions:

  • Furcellaran is a potent inhibitor of ice growth, offering cryoprotection similar to κ-carrageenan.
  • Optimal recrystallization inhibition by furcellaran is achieved at concentrations below those that induce gel formation.
  • Avoiding a gel-like consistency is crucial for maximizing furcellaran's efficacy in preserving biological materials during frozen storage.