Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Coaxial One-Dimensional Nanowires: Artificial Interface Engineering for Next-Generation Energy Storage.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Characterization of the gastric mucosal microbiota in tumoral and peritumoral mucosa in patients with advanced gastric cancer from Northwest China.

Frontiers in microbiology·2026

Same author

From Restoration to Regeneration: A Review of 3D Printing Strategies in Digital Dentistry.

Tissue engineering. Part B, Reviews·2026

Same author

Targeting ATF4 overcomes dual resistance to chemo-immunotherapy in gastric cancer by disrupting the DNA damage response.

Journal for immunotherapy of cancer·2026

Same author

An extensive forward genetic screen identifies the HWS-AGO1 axis as pivotal for target mimicry-mediated miRNA degradation in Arabidopsis.

The Plant journal : for cell and molecular biology·2026

Same author

Mechanistic insights into enhanced drought resistance of modern cultivars and drought-tolerance indicator screening.

Frontiers in plant science·2026

Search research articles

Related Experiment Video

Updated: Jun 21, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

Efficient and precise genomic deletion in rice using enhanced prime editing.

Mengyuan Liu¹, Xiang Zhang¹, Wen Xu¹

¹Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097 China.

|July 8, 2024

Summary

Prime editing-mediated deletion (PDel) enables precise genomic modifications in plants. This study introduces PDel and PDel/Syn strategies for efficient and accurate gene deletions, advancing plant research and crop improvement.

Keywords:

CRISPR Cas9 Precise genomic deletion Prime editing

More Related Videos

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

Author Spotlight: Advancing Gene Editing in Bamboo Leaves for Sustainable Plastic Alternatives

Author Spotlight: Advancing Gene Editing in Bamboo Leaves for Sustainable Plastic Alternatives

Published on: August 18, 2023

Related Experiment Videos

Last Updated: Jun 21, 2025

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

Author Spotlight: Advancing Gene Editing in Bamboo Leaves for Sustainable Plastic Alternatives

Author Spotlight: Advancing Gene Editing in Bamboo Leaves for Sustainable Plastic Alternatives

Published on: August 18, 2023

Area of Science:

Plant Genomics
Molecular Biology
Biotechnology

Background:

Precise genomic deletion is crucial for understanding protein function in plants.
Enhancing agricultural traits through genetic modification requires efficient gene editing tools.

Purpose of the Study:

To evaluate the PRIME-Del (PDel) strategy for genomic deletions in plants.
To develop and test the PDel/Syn strategy for improved deletion accuracy and efficiency.

Main Methods:

Utilized a pair of prime editing guide RNAs (pegRNAs) targeting opposite DNA strands for deletion.
Applied the PDel strategy for small (60 bp) and large (2000 bp) fragment deletions.
Developed the PDel/Syn strategy incorporating synonymous mutations to optimize repair.

Main Results:

Achieved an average deletion efficiency of 55.8% for 60 bp fragments using PDel.
Demonstrated high precise deletion efficiency (84.2%) for a 2000 bp deletion at the OsGS1 locus in rice.
The PDel/Syn strategy showed improved average deletion efficiency (58.1%) compared to PDel.

Conclusions:

The PDel strategy offers an efficient method for precise genomic deletions in plants.
The PDel/Syn strategy enhances deletion efficiency by managing unintentional sequence loss.
These strategies provide accurate and flexible tools for genetic engineering in rice and other plants.