A dual-labeling fluorescent probe to track lysosomal polarity and endoplasmic reticulum dynamics during ferroptosis
Zhao Zhao1, Wendong Jin2, Mengfan Wu3
1Research Center of Analytical Instrumentation, Key Laboratory of Bio-resource and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610065, Sichuan, China. zhaoz512@163.com.
Chemical Communications (Cambridge, England)
|July 8, 2024
Abstract:
A polarity-sensitive probe was developed to simultaneously label lysosomes and endoplasmic reticulum (ER) via its dansylamide and rhodamine fluorescence, respectively, enabling ratiometric polarity detection and stable dual-labeling. The fragmented ER network and increased lysosomal polarity during ferroptosis were revealed, which facilitates the understanding of ferroptotic mechanisms.
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