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The expression of MIR125B transcripts and bone phenotypes in Mir125b2-deficient mice
Tomohiro Ogasawara1, Shota Ito1, Shintaro Ogashira1
1Department of Orthodontics, Division of Oral Health and Development, Hiroshima University Hospital, Hiroshima, Japan.
Plos One
|July 8, 2024
Summary
MicroRNA 125B (MIR125B) plays a role in bone cell development. Deleting Mir125b2 in mice did not affect bone parameters, highlighting MIR125B-5p from Mir125b1 as key for bone biology.
Area of Science:
- Molecular Biology
- Genetics
- Developmental Biology
Background:
- MicroRNA 125B (MIR125B) is implicated in bone cell differentiation.
- MIR125B is transcribed from Mir125b1 and Mir125b2 loci, yielding multiple transcripts.
- Expression profiles and bone-specific roles of MIR125B transcripts are not well understood.
Purpose of the Study:
- To investigate the role of Mir125b2 in MIR125B expression and bone phenotypes.
- To determine the contribution of Mir125b2 to bone development and homeostasis.
Main Methods:
- Generation of Mir125b2 knockout (KO) mice.
- Analysis of MIR125B primary and mature transcript expression in various tissues.
- Assessment of bone parameters in wild-type (WT) and Mir125b2 KO mice.
Main Results:
- Primary and mature MIR125B transcripts showed differential expression across tissues.
- Mir125b2 KO mice exhibited normal growth and bone parameters.
- MIR125B-5p was abundant in calvaria and bone marrow stromal cells in both WT and KO mice.
Conclusions:
- Genetic ablation of Mir125b2 does not significantly impact bone parameters in mice.
- MIR125B-5p, likely originating from Mir125b1, warrants further investigation for its role in bone biology.
- Future studies should explore conditional deletion of Mir125b1 and both loci.
