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Sample Preparation for Analysis: Overview

Sample Preparation for Analysis: Overview

Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...

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Updated: May 5, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

Detailed Protocol for Solid-Phase Extraction for Lipidomic Analysis.

Marian N Aziz¹, Leticia Brotto², Ahmed S Yacoub²

¹Cyclotron and Radiochemistry Program, Radiology Department, University of Texas Southwestern Medical Center, Dallas, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)

|July 8, 2024

Summary

This summary is machine-generated.

Solid-Phase Extraction (SPE) is crucial for lipidomic analysis, enabling precise purification of lipids from biological samples like serum, plasma, and tissues. Optimizing SPE involves careful selection of materials, flow rate, and storage conditions for reliable results.

Keywords:

SPE for lipidomic SPE for muscle SPE for plasma SPE for serum Sample preparation

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Last Updated: May 5, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

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Published on: November 17, 2011

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils

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Published on: August 26, 2016

The MPLEx Protocol for Multi-omic Analyses of Soil Samples

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Published on: May 30, 2018

Area of Science:

Biochemistry
Analytical Chemistry
Omics Sciences

Background:

Robust analytical techniques are essential for understanding cellular functions through omic profiling.
Precise sample preparation is critical for comprehensive analysis of biological molecules.
Lipidomics requires effective methods for extracting and purifying lipids from complex biological matrices.

Purpose of the Study:

To detail Solid-Phase Extraction (SPE) methodologies for lipidomic analysis.
To highlight the importance of SPE in preparing biofluid and tissue samples for lipidomic studies.
To discuss key factors influencing the success of SPE in lipidomic applications.

Main Methods:

Solid-Phase Extraction (SPE) as the primary technique for lipid isolation.
Application of SPE to biofluid samples (serum and plasma).
Application of SPE to muscle tissue samples.

Main Results:

SPE is an indispensable, user-friendly method for lipid extraction and purification.
Key factors influencing SPE reliability include column material, flow rate, and storage.
Detailed SPE protocols are provided for specific biological sample types.

Conclusions:

SPE is a vital technique for advancing lipidomic research.
Optimized SPE protocols enhance the accuracy and reliability of lipidomic analyses.
This chapter provides practical guidance for performing SPE in lipidomics.