Related Experiment Video
Updated: Jun 21, 2025
10:24
Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
Published on: March 11, 2018
6.3K
Dietary restriction promote sperm remodeling in aged roosters based on transcriptome analysis
Wenjie Liang1, Yuehua He1, Tingqi Zhu1
1College of Animal Science and Technology, Henan Agricultural University, Ping'an Avenue 218#, Zhengdong New District, Zhengzhou, 450046, P. R. China.
BMC Genomics
|July 8, 2024
Summary
Induced molting in roosters improved sperm quality and testis weight. Transcriptome sequencing identified 61 differentially expressed genes, including WNT5A and FGFR3, potentially enhancing fertility in aging roosters.
Area of Science:
- Poultry Science
- Reproductive Biology
- Genomics
Background:
- Aging breeder roosters experience declining fertility, impacting poultry production.
- Improving fertility in older roosters is crucial for extending their productive lifespan.
- Current methods for enhancing rooster fertility face significant challenges.
Purpose of the Study:
- To identify genes associated with improved sperm remodeling in aged Houdan roosters.
- To analyze the correlation between semen quality and testis development using transcriptome sequencing.
- To provide data for enhancing the reproductive value of aging roosters.
Main Methods:
- Selected 350-day-old Houdan roosters undergoing induced molting (D group) and non-induced molting (47DG group).
- Performed transcriptome sequencing (RNA-seq) on testis tissues to analyze differentially expressed genes (DEGs).
- Utilized quantitative real-time PCR (qPCR) to validate the expression of selected DEGs.
Main Results:
- Induced molting significantly increased sperm motility, sperm density, and testis weight in roosters.
- RNA-seq identified 61 DEGs (21 up-regulated, 40 down-regulated) between the groups.
- DEGs were enriched in pathways including cytokine-cytokine receptor interaction, Wnt signaling, and MAPK signaling.
- Six genes (WNT5A, FGFR3, AGTR2, TGFβ2, ROMO1, SLC26A7) were identified as potentially playing roles in testis development and spermatogenesis.
Conclusions:
- Induced molting positively impacts sperm quality and testis development in aging roosters.
- Specific genes identified may be key targets for improving rooster fertility.
- This research offers foundational data for enhancing the reproductive efficiency of aging poultry.
