Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 21, 2025

Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos

Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos

Published on: February 19, 2016

Zebrafish do not have calprotectin.

Kona N Orlandi, Michael J Harms

Biorxiv : the Preprint Server for Biology

|July 9, 2024

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Navigating contradictions in enteric chemotactic stimuli.

eLife·2025

Same author

Applying Computational Protein Design to Engineer Affibodies for Affinity-controlled Delivery of Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor.

Biomacromolecules·2025

Same author

Zebrafish do not have a calprotectin ortholog.

PloS one·2025

Same author

Changing expression system alters oligomerization and proinflammatory activity of recombinant human S100A9.

bioRxiv : the preprint server for biology·2024

Same author

Bacterial vampirism mediated through taxis to serum.

eLife·2024

Same author

S100A9 interacts with a dynamic region on CD14 to activate Toll-like receptor 4.

bioRxiv : the preprint server for biology·2024

Same journal

Layered social competition coordinates reproductive hierarchy formation in ants.

bioRxiv : the preprint server for biology·2026

Same journal

Combination epigenetic-targeted therapy increases the immunogenicity of poorly immunogenic sarcomas.

bioRxiv : the preprint server for biology·2026

Same journal

Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.

bioRxiv : the preprint server for biology·2026

Same journal

Integrative Transfer Network: Deep Transfer Learning Across Populations and Prediction Targets.

bioRxiv : the preprint server for biology·2026

Same journal

Confidence-supported label-free metabolic imaging with FPhaS phase autofluorescence microscopy.

bioRxiv : the preprint server for biology·2026

Same journal

Sequence-encoded autoinhibition couples mRNA decapping activity to phase separation.

bioRxiv : the preprint server for biology·2026

See all related articles

Zebrafish lack calprotectin, a key immune protein found in mammals. Researchers found no zebrafish orthologs of S100A8 or S100A9, and candidate proteins showed no comparable antimicrobial or anti-inflammatory functions.

Area of Science:

Immunology
Innate Immunity
Comparative Genomics

Background:

Calprotectin, a mammalian protein heterodimer (S100A8/S100A9), is crucial for innate immunity, exhibiting antibacterial and anti-inflammatory functions.
Understanding calprotectin's regulation and function aids in diagnostics, therapy, and comprehending host-microbe interactions.
Previous studies suggested zebrafish s100a10b as a potential calprotectin analog due to sequence and expression similarities.

Purpose of the Study:

To investigate whether zebrafish possess a functional ortholog of mammalian calprotectin.
To identify and characterize zebrafish S100 proteins with potential calprotectin-like roles in innate immunity.
To determine if any zebrafish S100 proteins exhibit antimicrobial or anti-inflammatory activities.

Main Methods:

More Related Videos

Using Alizarin Red Staining to Detect Chemically Induced Bone Loss in Zebrafish Larvae

Using Alizarin Red Staining to Detect Chemically Induced Bone Loss in Zebrafish Larvae

Published on: December 28, 2021

Development of a Larval Zebrafish Infection Model for Clostridioides difficile

Development of a Larval Zebrafish Infection Model for Clostridioides difficile

Published on: February 14, 2020

Related Experiment Videos

Last Updated: Jun 21, 2025

Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos

Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos

Published on: February 19, 2016

Using Alizarin Red Staining to Detect Chemically Induced Bone Loss in Zebrafish Larvae

Using Alizarin Red Staining to Detect Chemically Induced Bone Loss in Zebrafish Larvae

Published on: December 28, 2021

Development of a Larval Zebrafish Infection Model for Clostridioides difficile

Development of a Larval Zebrafish Infection Model for Clostridioides difficile

Published on: February 14, 2020

Comparative genomic analysis to identify zebrafish orthologs of mammalian S100A8 and S100A9.
Identification and selection of candidate zebrafish S100 proteins expressed in immune cells and upregulated during infection.
Recombinant expression, purification, and functional characterization of selected zebrafish S100 proteins for antimicrobial and proinflammatory activities.

Main Results:

Zebrafish do not possess orthologs of mammalian S100A8 or S100A9.
Four zebrafish S100 proteins, including s100a10b, were identified in immune cells and upregulated during immune responses.
None of the tested zebrafish S100 proteins demonstrated antimicrobial or anti-inflammatory activities comparable to mammalian calprotectin.

Conclusions:

Zebrafish lack a direct ortholog of mammalian calprotectin.
The identified candidate zebrafish S100 proteins have not convergently evolved similar immune functions to mammalian calprotectin.
This study clarifies the absence of calprotectin in zebrafish, impacting future research on innate immunity models.