Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

386
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
386
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

274
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
274
Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

496
Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
496
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

576
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
576

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>C</i>5-alkyl and <i>C</i>5-aryl Substituted 5-Deazaflavin as Sensitizers for Photodehalogenation of Aryl Halides.

Molecules (Basel, Switzerland)·2026
Same author

Carboxyl-functionalized flavin as efficient heavy-atom-free triplet photosensitizers: a theoretical investigation.

Physical chemistry chemical physics : PCCP·2026
Same author

Ni redispersion from SiO<sub>2</sub> to molybdenum carbide creates dual interfaces to boost tandem CO<sub>2</sub> hydrogenation.

Nature communications·2025
Same author

Principles of Synthesizing Transition Metal Silicate Hydroxides for Catalyzing the Oxygen Evolution Reaction.

Inorganic chemistry·2025
Same author

Study of the thermally-activated delayed fluorescence (TADF) mechanism of phenothiazine-dibenzothiophene-<i>S</i>,<i>S</i>-dioxide electron donor-acceptor dyads using steady-state and time-resolved optical and electron paramagnetic resonance spectroscopies.

Chemical science·2025
Same author

Ambidextrous fine-tuning of zeolite-like hydrogen-bonded organic frameworks (HOFs) <i>via</i> scalable green synthesis for efficient biogas upgrading.

Chemical science·2025
Same journal

Investigating the stabilizing influences of organic sidechains in epoxides by rotational spectroscopy and computational chemistry: 1,2-epoxyhexane <i>versus</i> 1,2-epoxy-5-hexene.

Physical chemistry chemical physics : PCCP·2026
Same journal

Structure and dynamics of water confined in graphene oxide.

Physical chemistry chemical physics : PCCP·2026
Same journal

Comprehensive first-principles study of group IIIA-VIA A<sub>2</sub>B<sub>3</sub> two-dimensional ferroelectrics.

Physical chemistry chemical physics : PCCP·2026
Same journal

Low-resistance ohmic contacts for NbGeN<sub>3</sub> enabled by cold metals.

Physical chemistry chemical physics : PCCP·2026
Same journal

Integrated first-principles, SCAPS-1D, and a ML framework for numerical modelling and performance optimization of BaXS<sub>3</sub> (X = Zr, Hf)-based perovskite solar cells.

Physical chemistry chemical physics : PCCP·2026
Same journal

Generalized Suzuki-Chin factorization in bosonic path integral molecular dynamics.

Physical chemistry chemical physics : PCCP·2026
See all related articles

Related Experiment Video

Updated: Jun 21, 2025

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
09:46

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Published on: August 19, 2013

15.6K

Lightening flavin by amination for fluorescent sensing.

Huimin Guo1, Siyu Liu1, Xin Liu1

  • 1School of Chemistry, Dalian University of Technology, No. 2, Linggong Road, Dalian, 116024, P. R. China. guohm@dlut.edu.cn.

Physical Chemistry Chemical Physics : PCCP
|July 9, 2024
PubMed
Summary
This summary is machine-generated.

Aminoflavins (AmFLs) show significantly enhanced fluorescent emission when modified at the C8 position. This makes 8-aminoflavins promising fluorescent sensors for monitoring reactive oxygen species (ROS) in biological systems.

More Related Videos

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons
11:35

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons

Published on: October 29, 2011

12.8K
Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One &#945;-Synuclein Monomer at a Time
07:56

Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time

Published on: May 30, 2021

3.2K

Related Experiment Videos

Last Updated: Jun 21, 2025

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
09:46

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores

Published on: August 19, 2013

15.6K
Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons
11:35

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons

Published on: October 29, 2011

12.8K
Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One &#945;-Synuclein Monomer at a Time
07:56

Utilizing Time-Resolved Protein-Induced Fluorescence Enhancement to Identify Stable Local Conformations One α-Synuclein Monomer at a Time

Published on: May 30, 2021

3.2K

Area of Science:

  • Photochemistry
  • Biophysical Chemistry
  • Molecular Spectroscopy

Background:

  • Reactive oxygen species (ROS) play crucial roles in biological processes but are implicated in various diseases when overproduced.
  • Flavin (FL) derivatives can serve as fluorescent sensors for ROS, as their fluorescence is quenched upon reduction.
  • Understanding the photophysical properties of flavin derivatives is key to developing sensitive ROS detection methods.

Purpose of the Study:

  • To theoretically investigate the effect of amino substitution on the photophysical properties of aminoflavins (AmFLs).
  • To identify structural modifications that enhance fluorescent emission for potential ROS sensing applications.

Main Methods:

  • Computational theoretical investigation of aminoflavin (AmFL) derivatives.
  • Analysis of electronic and positional effects on photophysical properties.
  • Estimation of fluorescent emission intensity and comparison with parent flavin.

Main Results:

  • Amination at the C8 position (8AmFL) significantly enhances fluorescent emission compared to unsubstituted flavin (FL).
  • C8 amination increases electronic coupling and emission transition dipole moments, while weakening vibronic coupling.
  • The fluorescent emission intensity of 8AmFL was theoretically estimated to be approximately 40 times greater than that of FL.

Conclusions:

  • The C8-aminated flavin derivative (8AmFL) exhibits drastically enhanced fluorescence.
  • 8AmFL shows significant potential as a highly sensitive fluorescent sensor for monitoring reactive oxygen species (ROS).
  • Theoretical insights guide the design of advanced fluorescent probes for biological applications.