Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

AI in Atomic Force Microscopy: Advancing Biological Nanoscale Imaging and Autonomous Discovery.

ACS nano·2026

Same author

Defect-Related Photoluminescence in Hydroxyapatite Nanoparticles Modulated by Carbonate Incorporation.

ACS nanoscience Au·2026

Same author

Rapid desiccation and on-disc rehydration of extracellular vesicles for non-cryogenic preservation.

Lab on a chip·2026

Same author

Automated disc device for multiplexed extracellular vesicle isolation and labelling from liquid biopsies in cancer diagnostics.

Nature biomedical engineering·2026

Same author

Hypoxia induced VEGF secretion promotes resistance to bispecific T-cell engagers.

Signal transduction and targeted therapy·2025

Same author

Semi-Quantitative On-Site Microfluidic Assay to Detect 11-Nor-9-carboxy-delta 9-Tetrahydrocannabinol (THC-COOH) in Urine.

Sensors (Basel, Switzerland)·2025

Search research articles

Related Experiment Video

Updated: Jun 21, 2025

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

Upconverting Nanoparticle-based Enhanced Luminescence Lateral-Flow Assay for Urinary Biomarker Monitoring.

Marylyn Setsuko Arai^1,2, Hyunho Kim², Madeleine Pascavis²

¹São Carlos Institute of Physics, University of São Paulo, São Carlos, SP 13566-590, Brazil.

ACS Applied Materials & Interfaces

|July 9, 2024

Summary

This study presents a novel upconverting nanoparticle (UCNP) based lateral-flow assay for rapid and accurate detection of kidney injury biomarkers. The enhanced sensor platform offers a user-friendly solution for early disease diagnosis.

Keywords:

biosensor kidney injury lateral flow portable sensor upconverting nanoparticles

More Related Videos

Estimation of Urinary Nanocrystals in Humans using Calcium Fluorophore Labeling and Nanoparticle Tracking Analysis

Estimation of Urinary Nanocrystals in Humans using Calcium Fluorophore Labeling and Nanoparticle Tracking Analysis

Published on: February 9, 2021

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

Related Experiment Videos

Last Updated: Jun 21, 2025

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

Estimation of Urinary Nanocrystals in Humans using Calcium Fluorophore Labeling and Nanoparticle Tracking Analysis

Estimation of Urinary Nanocrystals in Humans using Calcium Fluorophore Labeling and Nanoparticle Tracking Analysis

Published on: February 9, 2021

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

Area of Science:

Nanotechnology
Biomedical Engineering
Analytical Chemistry

Background:

Accurate and early disease diagnosis relies on efficient biomarker detection, a persistent challenge in portable sensing.
Existing diagnostic methods often lack the speed, sensitivity, or portability required for widespread clinical application.

Purpose of the Study:

To develop an enhanced luminescence lateral-flow assay using upconverting nanoparticles (UCNPs) for sensitive and rapid biomarker detection.
To demonstrate the sensor's capability for kidney health monitoring by detecting acute kidney injury biomarkers.

Main Methods:

Engineered multi-layered upconverting nanoparticles (UCNP@mSiO2@Au) with enhanced luminescence properties.
Integrated UCNPs into a lateral-flow assay platform with a portable reader and smartphone app.
Quantified kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) in urine samples.

Main Results:

The UCNP@mSiO2@Au nanoparticles exhibited over 40-fold enhanced emission.
The assay successfully detected KIM-1 and NGAL in urine samples within the range of 0.1 to 20 ng/mL.
Achieved low limits of detection (0.28 ng/mL for KIM-1, 0.23 ng/mL for NGAL) and results correlated well with ELISA.

Conclusions:

The developed UCNP-based lateral-flow assay provides a reliable, rapid, and user-friendly diagnostic tool.
This technology holds significant potential for early disease diagnosis and point-of-care applications.
The enhanced luminescence and ease of use make the UCNP platform versatile for various bioassays.