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Cardiac Action Potential

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Related Experiment Video

Updated: Jun 21, 2025

Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique

Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique

Published on: November 11, 2022

Bionic Potassium Ion Channel in Live Cells Repairs Cardiomyocyte Function.

Xuejie Shen¹, Qingqing Lu², Tianhuan Peng¹

¹Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China.

Journal of the American Chemical Society

|July 10, 2024

Summary

Researchers developed a vesicle fusion-based bionic porin (VFBP) to integrate artificial potassium ion channels into cardiac cells, restoring normal function and offering potential treatments for cardiovascular diseases.

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Last Updated: Jun 21, 2025

Voltage-Dependent Potassium Current Recording on H9c2 Cardiomyocytes via the Whole-Cell Patch-Clamp Technique

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Published on: November 11, 2022

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Published on: June 4, 2017

Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples

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Published on: April 21, 2014

Area of Science:

Biomedical Engineering
Cardiovascular Research
Nanotechnology

Background:

Potassium channel dysfunction in cardiac myocytes causes electrophysiological disorders and cardiovascular diseases.
Artificial potassium ion channels offer a potential therapeutic strategy for channelopathies.
Introducing bionic channels into cells faces challenges in controlling membrane insertion angle.

Purpose of the Study:

To develop a method for integrating bionic potassium ion channels into cardiomyocytes.
To overcome the challenge of controlling bionic channel insertion angle during cell membrane fusion.
To restore normal cardiac myocyte function using vesicle fusion-based bionic porins.

Main Methods:

Development of a vesicle fusion-based bionic porin (VFBP) system.
Integration of bionic potassium ion channels into cardiomyocytes via vesicle fusion.
Theoretical and experimental validation of channel function and cellular repair.

Main Results:

VFBP successfully integrated bionic potassium ion channels into cardiomyocytes.
Inserted bionic channels exhibited potassium ion transport rates comparable to natural channels.
Restoration of potassium ion outflow, abnormal action potential, and excitation-contraction coupling in cardiomyocytes.

Conclusions:

Vesicle fusion-based bionic porins provide a viable method for introducing functional bionic ion channels into living cells.
This approach holds promise for treating channelopathies and advancing ultrafast ion transport and transmembrane delivery.
The bionic potassium channel system offers a novel therapeutic avenue for cardiovascular diseases stemming from potassium channel dysfunction.