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  2. Intensity-based Optoretinography Reveals Sub-clinical Deficits In Cone Function In Retinitis Pigmentosa.
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  2. Intensity-based Optoretinography Reveals Sub-clinical Deficits In Cone Function In Retinitis Pigmentosa.

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Intensity-based optoretinography reveals sub-clinical deficits in cone function in retinitis pigmentosa.

Mina Gaffney1, Thomas B Connor2, Robert F Cooper1,2

  • 1Joint Department of Biomedical Engineering, Marquette University and the Medical College of Wisconsin, Milwaukee, WI, United States.

Frontiers in Ophthalmology
|July 10, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

Intensity based optoretinography (iORG) using adaptive optics scanning light ophthalmoscopy (AOSLO) detects early photoreceptor function deficits in retinitis pigmentosa (RP). iORG shows greater sensitivity to sub-clinical changes than traditional methods, aiding early diagnosis and therapy identification.

Keywords:
adaptive optics scanning light ophthalmoscopycone photoreceptorsmicroperimetryoptoretinographyretinitis pigmentosa

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Area of Science:

  • Ophthalmology
  • Cellular Biology
  • Medical Imaging

Background:

  • Clinical tools for retinitis pigmentosa (RP) diagnosis often lack sensitivity, missing early photoreceptor disruption.
  • Adaptive optics scanning light ophthalmoscopy (AOSLO) offers high-resolution imaging for detailed structural and functional assessment of photoreceptors.

Purpose of the Study:

  • To evaluate intensity based optoretinography (iORG) via AOSLO for assessing photoreceptor structure and function in individuals with and without RP.
  • To compare the sensitivity of iORG to traditional methods like macular integrity assessment (MAIA) for detecting early functional deficits.

Main Methods:

  • AOSLO was used to acquire iORG in 15 healthy individuals and 7 with RP.
  • Photoreceptor structure was quantified by cone nearest neighbor distance (NND) and cone outer segment length (measured via OCT-derived LRPs).
  • iORG functional measures were compared with MAIA microperimeter retinal sensitivity.
  • Main Results:

    • Individuals with RP showed increased NND and decreased outer segment length with eccentricity.
    • RP participants exhibited reduced iORG amplitudes compared to controls, with greater reduction in peripheral retina.
    • iORG detected earlier functional deficits than MAIA, which was more sensitive to later disease stages.

    Conclusions:

    • iORG is a valuable tool for detecting sub-clinical cone dysfunction across all stages of RP.
    • iORG's sensitivity supports its future use in identifying cells for targeted cellular therapies in RP.