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Updated: Jun 21, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Gene Editing with Artificial DNA Scissors.

Alex Gibney¹, Andrew Kellett¹

¹SSPC, The Science Foundation Ireland Research Centre for Pharmaceuticals, School of Chemical Sciences, Dublin City University, Glasnevin, Dublin, 9, Ireland.

Chemistry (Weinheim an Der Bergstrasse, Germany)

|July 10, 2024

Summary

Artificial metallo-nucleases (AMNs) are DNA-cleaving agents with therapeutic potential. This review explores AMNs, their DNA interactions, and hybrid designs for enhanced gene editing applications.

Keywords:

Chemical nucleases Click chemistry Conjugation strategies DNA damage Metal complexes Nucleic acids

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Area of Science:

Biochemistry
Molecular Biology
Medicinal Chemistry

Background:

Artificial metallo-nucleases (AMNs) function as DNA molecular scissors.
AMNs are a promising class of chemotherapeutic agents.
Understanding DNA structure, function, and repair is crucial for AMN development.

Purpose of the Study:

To introduce fundamental concepts of DNA and AMNs.
To explore hybrid AMN designs with enhanced DNA cleavage capabilities.
To discuss synthetic strategies and future applications of AMNs in gene editing.

Main Methods:

Review of existing literature on DNA structure, function, damage, and repair.
Exploration of AMN chemistry and design principles.
Analysis of conjugation strategies for creating hybrid AMNs.

Main Results:

AMNs offer sequence-specific DNA cleavage.
Hybrid molecules demonstrate enhanced DNA damaging capabilities.
Synthetic conjugation provides a basis for novel AMN designs.

Conclusions:

AMNs hold significant clinical and therapeutic potential.
Hybrid AMNs can be engineered for targeted DNA modification.
Future innovations may arise from advanced AMN design for artificial gene editing systems.