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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Related Experiment Video

Updated: Jan 9, 2026

Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC Crosslinking of Small Molecules to Isolate Chromatin
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Recent progress in probing small molecule interactions with DNA.

Simon Poole1, Bríonna McGorman1, Christine J Cardin2

  • 1School of Chemical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.

Biophysical Reviews
|December 11, 2025
PubMed
Summary

Understanding drug-DNA interactions with non-canonical nucleic acids is key for developing targeted therapies. Recent advancements in biophysical techniques aid in discovering new clinical agents for gene regulation and genome stability.

Keywords:
B-DNADNADrug-DNA interactionsHolliday junctionNon-canonical DNANucleic acidsThree-way junctionTriple helixTriplex-forming oligonucleotides

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Area of Science:

  • Medicinal Chemistry
  • Molecular Biology
  • Biophysics

Background:

  • Nucleic acids are crucial therapeutic targets.
  • Developing targeted therapies requires understanding drug-DNA interactions.
  • Non-canonical nucleic acid structures (e.g., G-quadruplexes, i-motifs) play roles in gene regulation and genome stability.

Purpose of the Study:

  • To review recent advancements in analyzing higher-order drug-DNA interactions.
  • To highlight the role of these interactions in the rational design of targeted therapeutics.
  • To discuss the therapeutic potential of ligands and metal complexes interacting with non-canonical nucleic acids.

Main Methods:

  • Spectroscopic methods
  • Crystallography
  • Biomolecular assays
  • Analysis of drug-DNA interactions

Main Results:

  • New molecules preferentially interact with complex nucleic acid structures.
  • Characterizing interactions with non-canonical structures has identified promising therapeutic agents.
  • Biophysical techniques are critical for probing these interactions.

Conclusions:

  • Advancements in analyzing drug-DNA interactions facilitate the design of targeted therapeutics.
  • Targeting non-canonical nucleic acids offers potential for novel clinical agents.
  • Understanding these interactions is essential for drug discovery.