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Updated: May 1, 2026

Transcriptome Analysis of Single Cells

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

Unraveling plant-microbe symbioses using single-cell and spatial transcriptomics.

Karen Serrano¹, Francesca Tedeschi², Stig U Andersen²

¹Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, CA 94720, USA; DOE Joint BioEnergy Institute, 5885 Hollis Street, Emeryville, CA 94608, USA; Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720, USA.

Trends in Plant Science

|July 11, 2024

Summary

Single-cell and spatial transcriptomics offer new ways to study plant-microbe symbioses. These advanced methods reveal symbiotic interactions in detail, overcoming limitations of older techniques.

Keywords:

high-throughput sequencing mycorrhizae rhizobia single-cell RNA-seq spatial transcriptomics symbiosis

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Isolation and Transcriptome Analysis of Plant Cell Types

Isolation and Transcriptome Analysis of Plant Cell Types

Published on: April 7, 2023

Area of Science:

Plant biology
Microbiology
Genomics

Background:

Plant-microbe symbioses involve complex genetic coordination and cell-type-specific interactions.
Traditional RNA sequencing lacks the cellular resolution to fully understand these symbiotic processes.

Purpose of the Study:

To discuss the advantages of single-cell and spatial transcriptomics for studying plant-microbe endosymbioses.
To highlight recent advancements and remaining challenges in applying these technologies to symbiosis research.

Main Methods:

Utilizing single-cell transcriptomics (scRNA-seq) to analyze gene expression at the individual cell level.
Employing spatial transcriptomics (ST) to map gene expression within the tissue context of symbiotic interactions.

Main Results:

Single-cell and spatial transcriptomics provide unprecedented cellular resolution for studying symbiotic interactions.
These technologies enable detailed analysis of host and microbial gene expression within specific cell types.

Conclusions:

Novel transcriptomic approaches significantly enhance the study of plant-microbe symbioses.
Future research needs to address challenges in simultaneously capturing both plant and microbial transcripts within the same cells for a complete understanding.