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Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Published on: March 8, 2018
First draft genome sequence data of TA4-1, the type strain of Gram-positive bacterium Streptomyces chiangmaiensis
Montri Yasawong1,2, A'liyatur Rosyidah3, Thunwarat Songngamsuk1
1Programme on Environmental Toxicology, Chulabhorn Graduate Institute, Chulabhorn Royal Academy, Bangkok 10210, Thailand.
Abstract:
TA4-1 is the type strain of Streptomyces chiangmaiensis. The TA4-1 strain was isolated from a stingless bee (Tetragonilla collina). Here we present the draft genome sequence data of S. chiangmaiensis TA4-1. The Illumina NextSeq 550 sequencer was used to generate paired-end reads from the genomic DNA of the pure culture of S. chiangmaiensis TA4-1. The draft genome sequence of strain TA4-1 consists of 776 contigs with a total size of 9,707,984 base pairs, an N50 of 32,937 base pairs, and a GC content of 69.73 %. Digital DNA-DNA hybridisation (dDDH) and average nucleotide identity (ANI) analysis showed that S. yaanensis CGMCC 4.7035 had the highest dDDH value (32.7 %) and ANIm value (88.50 %) when compared with TA4-1. The presented data indicate the potential for a reference genome sequence in bacterial taxonomy, comparative genomics, and the investigation of bioactive compound biosynthesis in S. chiangmaiensis TA4-1. The draft genome sequence data have been deposited at NCBI under the Bioproject accession number PRJNA680432.
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