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Sensory Functions of the Skin

Sensory Functions of the Skin

The skin is the largest organ of the human body and plays a crucial role in our sensory perception. It contains a vast network of sensory receptors that contribute to the skin's protective function by perceiving physical, biological, and environmental cues and generating relevant responses.
There are two main categories of receptors on the skin: capsulated and non-capsulated. The non-capsulated ones are mainly the pain receptors. The capsulated ones can be further categorized based on the...

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Assessing Barrier Function in Psoriasis and Cornification Models of Artificial Skin Using Non-Invasive Impedance

Jaehwan Ahn¹, Yoon Sung Nam^1,2

¹Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|July 12, 2024

Summary

A new non-invasive impedance spectroscopy method quantifies stratum corneum (SC) and keratinocyte layer (KL) barrier function in reconstructed epidermal equivalents (REEs). This technique enables precise evaluation of skin barrier integrity and aids in understanding inflammatory skin disorders.

Keywords:

impedance spectroscopy psoriasis reconstructed epidermal equivalents skin barrier stratum corneum

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Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility

Published on: March 28, 2014

Area of Science:

Biophysics
Dermatology
Tissue Engineering

Background:

Reconstructed epidermal equivalents (REEs) comprise stratum corneum (SC) and keratinocyte layer (KL).
SC barrier defects are linked to immune dysregulation in inflammatory skin conditions like psoriasis.
Evaluating individual SC and KL barrier function in REEs is challenging due to a lack of non-disruptive quantitative methods.

Purpose of the Study:

To introduce a non-invasive impedance spectroscopy technique for dissecting SC and KL contributions to skin barrier function in REEs.
To establish a correlation between impedance parameters and specific skin layers using an equivalent circuit model.
To demonstrate the technique's ability to detect subtle barrier changes and characterize immature SC.

Main Methods:

Development and application of a non-invasive impedance spectroscopy technique.
Utilizing an equivalent circuit model to interpret impedance spectra.
Testing the method on REEs exposed to mild irritants and in psoriatic models.

Main Results:

The impedance spectroscopy technique successfully differentiates the barrier properties of SC and KL.
Individual barrier resistances and maturation levels of each layer were inferred from impedance spectra.
The method detected increased paracellular permeability from irritants and characterized immature SC in psoriatic models.

Conclusions:

This non-invasive impedance spectroscopy approach provides a quantitative method for assessing individual SC and KL barrier function in REEs.
The technique offers insights beyond traditional full-thickness skin analysis.
This research supports mechanistic investigations and therapeutic development for skin barrier disorders.