Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antibody Structure01:10

Antibody Structure

59.8K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
59.8K
Antibody Structure and Classes01:25

Antibody Structure and Classes

871
Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
871
Protein Organization01:13

Protein Organization

137.2K
Overview
137.2K
Peptide Bonds02:43

Peptide Bonds

74.1K
A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
74.1K
Protein and Protein Structure02:15

Protein and Protein Structure

79.4K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
79.4K
Antibody Actions01:26

Antibody Actions

1.1K
Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sharing big data for sustainable agri-food innovation.

Nature biotechnology·2026
Same author

AIntibody: an experimentally validated in silico antibody discovery design challenge.

Nature biotechnology·2024
Same author

DiscoTope-3.0: improved B-cell epitope prediction using inverse folding latent representations.

Frontiers in immunology·2024
Same author

Variability analysis of LC-MS experimental factors and their impact on machine learning.

GigaScience·2023
Same author

Comparative Structure Analysis of the Multi-Domain, Cell Envelope Proteases of Lactic Acid Bacteria.

Microorganisms·2023
Same author

Antioxidant peptides from alternative sources reduce lipid oxidation in 5% fish oil-in-water emulsions (pH 4) and fish oil-enriched mayonnaise.

Food chemistry·2023

Related Experiment Video

Updated: Jun 21, 2025

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

9.5K

Structural Characterization of Peptide Antibodies.

Anna Chailyan1, Paolo Marcatili2

  • 1Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.

Methods in Molecular Biology (Clifton, N.J.)
|July 12, 2024
PubMed
Summary

Synthetic peptides offer a versatile alternative to proteins for antibody production. This study introduces a pipeline to classify antibody binding sites and identify key residues influencing antigen interaction.

Keywords:
ClusteringLinear epitopePeptide antibodyStructure

More Related Videos

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
11:57

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions

Published on: April 21, 2016

6.7K
Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
07:26

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

12.9K

Related Experiment Videos

Last Updated: Jun 21, 2025

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

9.5K
Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
11:57

Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions

Published on: April 21, 2016

6.7K
Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides
07:26

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Published on: November 21, 2013

12.9K

Area of Science:

  • Immunology
  • Structural Biology
  • Biochemistry

Background:

  • Proteins are effective immunogens for antibody generation, but synthetic peptides provide a flexible alternative.
  • Synthetic peptides can be modified for various applications, including antibody production and protein quantitation.
  • Antibodies targeting synthetic peptides are crucial tools in biological research.

Purpose of the Study:

  • To present a novel computational pipeline for the structural classification of immunoglobulin antigen binding sites.
  • To identify key sequence residues and other variables critical for antibody-antigen interactions within different structural classes.

Main Methods:

  • Development of a computational pipeline for analyzing immunoglobulin structures.
  • Structural classification of antibody antigen binding sites.
  • Inference of critical sequence residues and molecular features.

Main Results:

  • A systematic classification of antibody antigen binding sites based on structural features.
  • Identification of sequence determinants associated with specific binding site classes.
  • Insights into the molecular basis of antibody-antigen recognition.

Conclusions:

  • The developed pipeline enables a deeper understanding of antibody structure-function relationships.
  • This classification aids in predicting and designing antibodies with desired binding properties.
  • The findings contribute to advancing antibody engineering and therapeutic development.